Knock-Out of CmNAC-NOR Affects Melon Climacteric Fruit Ripening.
Knock-Out of CmNAC-NOR Affects Melon Climacteric Fruit Ripening.
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敲除 CmNAC-NOR 影响甜瓜更年期果实成熟
DOI：
10.3389/fpls.2022.878037
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
果实成熟是影响果实品质的重要过程。在甜瓜中发现了一个参与更年期成熟调控的QTL ETHQV6.3，该QTL是由番茄NOR基因的同源基因CmNAC-NOR编码的。为了进一步研究CmNAC-NOR的功能，我们获得了两个CRISPR/ cas9介导的突变体（nor-3和nor-1）。nor-3含有一个3 bp的缺失，改变了NAC结构域a，导致成熟延迟~8天，但不影响果实品质。相比之下，nor-1基因中1 bp的缺失导致NAC结构域被完全破坏，从而完全阻断了更年期成熟。无1号果实不产生乙烯，不形成脱落层，外观颜色无变化。此外，挥发性成分发生了显著变化，种子发育不良，果肉硬度也发生了变化。非-1等位基因杂合后果实成熟延迟~20天。我们的研究结果为CmNAC-NOR在甜瓜果实成熟过程中的功能提供了新的信息，表明它是调节商品更年期甜瓜品种保质期的潜在靶点。
Fruit ripening is an important process that affects fruit quality. A QTL in melon, ETHQV6.3, involved in climacteric ripening regulation, has been found to be encoded by CmNAC-NOR, a homologue of the tomato NOR gene. To further investigate CmNAC-NOR function, we obtained two CRISPR/Cas9-mediated mutants (nor-3 and nor-1) in the climacteric Védrantais background. nor-3, containing a 3-bp deletion altering the NAC domain A, resulted in ~8 days delay in ripening without affecting fruit quality. In contrast, the 1-bp deletion in nor-1 resulted in a fully disrupted NAC domain, which completely blocked climacteric ripening. The nor-1 fruits did not produce ethylene, no abscission layer was formed and there was no external color change. Additionally, volatile components were dramatically altered, seeds were not well developed and flesh firmness was also altered. There was a delay in fruit ripening with the nor-1 allele in heterozygosis of ~20 days. Our results provide new information regarding the function of CmNAC-NOR in melon fruit ripening, suggesting that it is a potential target for modulating shelf life in commercial climacteric melon varieties.
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影响因子：
5.6
作者：
Adaskaveg JA;Silva CJ;Huang P;Blanco-Ulate B
通讯作者：
Blanco-Ulate B
影响因子：
5.4
作者：
Eduardo, I;Arús, P;Monforte, AJ
通讯作者：
Monforte, AJ
影响因子：
3
作者：
Garcia-Almodovar, R. C.;Gosalvez, B.;Burgos, L.
通讯作者：
Burgos, L.
影响因子：
5.1
作者：
El-Sharkawy, I;Manríquez, D;Pech, JC
通讯作者：
Pech, JC
NAC 转录因子 NOR-like1 是番茄果实成熟的新正调节因子
影响因子：
8.7
作者：
Gao, Ying;Wei, Wei;Fu, Da-Qi
通讯作者：
Fu, Da-Qi