Supplementation of a grape seed and grape marc meal extract decreases activities of the oxidative stress-responsive transcription factors NF-κB and Nrf2 in the duodenal mucosa of pigs.
Supplementation of a grape seed and grape marc meal extract decreases activities of the oxidative stress-responsive transcription factors NF-κB and Nrf2 in the duodenal mucosa of pigs.
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DOI：
10.1186/1751-0147-55-18
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发表时间：
2013-03-02
影响因子：
2.1
通讯作者：
Eder K
中科院分区：
文献类型：
--
作者：
Gessner DK;Fiesel A;Most E;Dinges J;Wen G;Ringseis R;Eder K
在猪中，通常观察到肠道感染和肠道疾病（如腹泻）的发生，特别是在断奶后。本研究探讨了葡萄籽和葡萄渣提取物（GSGME）作为膳食补充剂饲喂具有潜在抑制猪小肠炎症过程的可能性的假设，其通过调节NF-κB和Nrf 2的活性来实现，这是由于其高含量的黄酮类化合物。将24头6周龄的杂交猪随机分为2组，每组12头，饲喂营养充足的不含或含1%GSGME的饲料4周。与对照组相比，GSGME组猪十二指肠黏膜NF-κB和Nrf 2的转录激活水平以及这些转录因子的各种靶基因的表达水平均降低（P < 0.05）。两组间肝脏和血浆中α-生育酚和硫代巴比妥酸反应物质（TBARS）的浓度、血浆总抗氧化能力以及肝脏中NF-κB和Nrf 2靶基因的相对mRNA丰度均无差异。但饲喂GSGME的猪的绒毛高度与隐窝深度之比和增重与料肉比均高于对照猪（P < 0.05）。该研究表明，富含多酚的GSGME的膳食补充剂抑制猪的十二指肠粘膜中的NF-κB的活性，并且因此可能提供有用的膳食策略来抑制猪中经常发生的肠道炎症。饲喂GSGME不影响猪的维生素E状况和抗氧化系统，但改善了增重：饲料比。总的来说，该研究表明，富含多酚的植物提取物如GSGME可以作为猪营养的有用饲料补充剂，以保持动物健康和提高生产性能。
In pigs, enteric infections and the development of gut disorders such as diarrhoea are commonly observed, particularly after weaning. The present study investigated the hypothesis that feeding a grape seed and grape marc extract (GSGME) as a dietary supplement has the potential to suppress the inflammatory process in the small intestine of pigs by modulating the activities of NF-κB and Nrf2 due to its high content of flavonoids. Twenty-four crossbred, 6 weeks old pigs were randomly assigned to 2 groups of 12 animals each and fed nutritionally adequate diets without or with 1% GSGME for 4 weeks. Pigs administered GSGME had a lower transactivation of NF-κB and Nrf2 and a lower expression of various target genes of these transcription factors in the duodenal mucosa than control pigs (P < 0.05). Concentrations of α-tocopherol and thiobarbituric acid reactive substances (TBARS) in liver and plasma and total antioxidant capacity of plasma and relative mRNA abundances of NF-κB and Nrf2 target genes in the liver did not differ between the two groups. However, the ratio of villus height:crypt depth and the gain:feed ratio was higher in the pigs fed GSGME than in control pigs (P < 0.05). This study shows that dietary supplementation of a polyphenol rich GSGME suppresses the activity of NF-κB in the duodenal mucosa of pigs and thus might provide a useful dietary strategy to inhibit inflammation in the gut frequently occurring in pigs. Feeding GSGME did not influence vitamin E status and the antioxidant system of the pigs but improved the gain:feed ratio. In overall, the study suggests that polyphenol-rich plant extracts such GSGME could be useful feed supplements in pig nutrition, in order to maintain animal health and improve performance.
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影响因子：
5
作者：
Choi, EJ;Chee, KM;Lee, BH
通讯作者：
Lee, BH
影响因子：
4.2
作者：
Déprez, S;Brezillon, C;Scalbert, A
通讯作者：
Scalbert, A
DOI：
10.1111/j.1439-0396.2011.01222.x
发表时间：
2012-12-01
影响因子：
2.7
作者：
Gessner, D. K.;Ringseis, R.;Eder, K.
通讯作者：
Eder, K.
影响因子：
1.9
作者：
Frémont, L;Gozzelino, MT;Linard, A
通讯作者：
Linard, A
影响因子：
7.1
作者：
CASPARY, WF
通讯作者：
CASPARY, WF