Differential contribution of direct-developing and stem cell-derived melanocytes to the zebrafish larval pigment pattern.
Differential contribution of direct-developing and stem cell-derived melanocytes to the zebrafish larval pigment pattern.
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DOI：
10.1016/j.ydbio.2009.11.019
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发表时间：
2010-01-15
影响因子：
2.7
通讯作者：
Johnson, Stephen L.
中科院分区：
文献类型：
--
作者：
Hultman, Keith A.;Johnson, Stephen L.
成体干细胞参与胚胎生长的程度通常是不清楚的，因为通常没有可靠的标记物或测定法来确定细胞是否来源于成体干细胞，例如黑素细胞干细胞（MSC）。我们以前已经表明，两个谱系的黑素细胞可以有助于幼斑马鱼色素模式。胚胎首先发育出一种个体发育模式，主要由ErbB独立的、直接发育的黑色素细胞组成。在黑素细胞消融后，该群体可以在再生过程中被ErbB依赖性MSC衍生的群体取代。在这项研究中，我们开发了一种黑素细胞分化试验，与消融MSC的药物一起使用，以调查MSC衍生的黑素细胞是否有助于个体发育模式。我们发现，基本上所有的黑素细胞，发展前3 dpf产生的ErbB的独立，直接发展的人口。类似地，头部的晚期发育（3dpf后）黑素细胞也是ErbB独立的。相反，在侧纹和背纹中受精后三天发育的黑素细胞对ErbB抑制剂敏感，表明它们来自MSC。我们发现，黑素细胞再生突变体kitj1e99和skiv2l2j24e1，这是非常正常的整体个体发育模式，也缺乏MSC衍生的贡献的横向条纹。这一结果表明，这些突变的潜在再生缺陷是MSC调控的缺陷。我们认为，MSC的调节功能可能是在幼虫发育过程中的质量控制作用，除了其在成虫的幼虫再生和生长以及体内平衡中的既定作用。
The extent of adult stem cell involvement in embryonic growth is often unclear, as reliable markers or assays for whether a cell is derived from an adult stem cell, such as the Melanocyte Stem Cell (MSC), are typically not available. We have previously shown that two lineages of melanocytes can contribute to the larval zebrafish pigment pattern. The embryo first develops an ontogenetic pattern that is largely composed of ErbB-independent, direct-developing melanocytes. This population can be replaced during regeneration by an ErbB-dependent MSC-derived population following melanocyte ablation. In this study, we developed a melanocyte differentiation assay, used together with drugs that ablate the MSC, to investigate whether MSC-derived melanocytes contribute to the ontogenetic pattern. We found that essentially all melanocytes that develop before 3 dpf arise from the ErbB-independent, direct-developing population. Similarly, late-developing (after 3 dpf) melanocytes of the head are also ErbB independent. In contrast, the melanocytes that develop after three days postfertilization in the lateral and dorsal stripe are sensitive to ErbB inhibitor, indicating that they are derived from the MSC. We show that melanocyte regeneration mutants kitj1e99 and skiv2l2j24e1, that are grossly normal for the overall ontogenetic pattern, also lack the MSC-derived contribution to the lateral stripe. This result suggests that the underlying regeneration defect of these mutations is a defect in MSC regulation. We suggest that the regulative functions of the MSC may serve quality control roles during larval development, in addition to its established roles in larval regeneration and growth and homeostasis in the adult.
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影响因子：
4.5
作者：
Yang, Chao-Tsung;Hindes, Anna E.;Hultman, Keith A.;Johnson, Stephen L.
通讯作者：
Johnson, Stephen L.
影响因子：
6.5
作者：
Yang, CT;Sengelmann, RD;Johnson, SL
通讯作者：
Johnson, SL
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
6.5
作者：
OZEKI, H;ITO, S;HIROBE, T
通讯作者：
HIROBE, T
DOI：
10.1111/j.1600-0749.2006.00349.x
发表时间：
2006-12-01
期刊：
PIGMENT CELL RESEARCH
影响因子：
--
作者：
Zou, Jian;Beermann, Friedrich;Wei, Xiangyun
通讯作者：
Wei, Xiangyun