Fruit-surface flavonoid accumulation in tomato is controlled by a SlMYB12-regulated transcriptional network.
Fruit-surface flavonoid accumulation in tomato is controlled by a SlMYB12-regulated transcriptional network.
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DOI：
10.1371/journal.pgen.1000777
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发表时间：
2009-12
期刊：
影响因子：
4.5
通讯作者：
Aharoni A
中科院分区：
文献类型：
--
作者：
Adato A;Mandel T;Mintz-Oron S;Venger I;Levy D;Yativ M;Domínguez E;Wang Z;De Vos RC;Jetter R;Schreiber L;Heredia A;Rogachev I;Aharoni A
覆盖植物空气表面的角质层是一种独特的结构，在器官发育和抵御各种胁迫条件中起着关键作用。在生殖器官外表面研究的框架下，对番茄无色果皮突变体进行了详细的分析。突变体果皮缺乏黄酮类色素柚皮素查尔酮，这可能影响果皮角质层的特征和功能。大规模的代谢和转录谱分析揭示了对初级和次级代谢的广泛影响，主要与苯丙素的生物合成有关，特别是类黄酮。这些并不局限于果实或其发育的特定阶段，并表明y突变表型是由于调控基因的突变。事实上，表达分析表明，3个r2r3 - myb型转录因子在y突变果皮中显著下调。其中的SlMYB12被定位到先前显示含有y突变的番茄1号染色体上的基因组区域。鉴定出另一个与无色脱皮性状共分离的突变等位基因，特异性下调SlMYB12，并在突变背景下通过过度表达SlMYB12挽救y表型，证实了该调节因子的损伤是y表型的基础。因此，本研究为肉质果实角质层结构的研究提供了新的视角，并为阐明番茄果实角质层中黄酮类化合物积累的调控网络铺平了道路。陆地植物进化的一个重要步骤是在它们的外表面形成角质层。尽管角质层在器官发育和抵御各种压力方面起着关键作用，但人们对产生角质层的代谢途径的调节知之甚少。黄酮类化合物通常嵌入角质层中，被认为可以影响角质层结构的特性，并提供抗辐射和病原体的保护。类黄酮是人类饮食中不可或缺的一部分，可能是观察到的富含水果的饮食有益效果的原因。在这里，我们详细研究了番茄无色果皮突变体，它缺乏果实角质层的主要成分黄酮类色素柚皮素查尔酮。对该突变体的广泛转录和代谢物分析表明，SlMYB12是控制番茄皮中黄酮类化合物积累的转录网络的关键调节因子。此外，角质层黄酮类成分的变化使我们能够评估这些成分作为角质层屏障的重要性。最后，由于远东地区的大多数商业品种都是基于y遗传背景，y基因的发现也将有助于番茄育种计划。
The cuticle covering plants' aerial surfaces is a unique structure that plays a key role in organ development and protection against diverse stress conditions. A detailed analysis of the tomato colorless-peel y mutant was carried out in the framework of studying the outer surface of reproductive organs. The y mutant peel lacks the yellow flavonoid pigment naringenin chalcone, which has been suggested to influence the characteristics and function of the cuticular layer. Large-scale metabolic and transcript profiling revealed broad effects on both primary and secondary metabolism, related mostly to the biosynthesis of phenylpropanoids, particularly flavonoids. These were not restricted to the fruit or to a specific stage of its development and indicated that the y mutant phenotype is due to a mutation in a regulatory gene. Indeed, expression analyses specified three R2R3-MYB–type transcription factors that were significantly down-regulated in the y mutant fruit peel. One of these, SlMYB12, was mapped to the genomic region on tomato chromosome 1 previously shown to harbor the y mutation. Identification of an additional mutant allele that co-segregates with the colorless-peel trait, specific down-regulation of SlMYB12 and rescue of the y phenotype by overexpression of SlMYB12 on the mutant background, confirmed that a lesion in this regulator underlies the y phenotype. Hence, this work provides novel insight to the study of fleshy fruit cuticular structure and paves the way for the elucidation of the regulatory network that controls flavonoid accumulation in tomato fruit cuticle. A major step in the evolution of land plants was the formation of a cuticular layer on their outer surfaces. Despite the cuticle's key role in organ development and in protecting against a variety of stresses, very little is known about the regulation of the metabolic pathways that generate its building blocks. Flavonoids, often embedded in the cuticle, have been suggested to impact the characteristics of this structure and to provide protection against radiation and pathogens. Flavonoids are an integral part of the human diet and are likely responsible for the observed beneficial effects of a fruit-rich diet. Here, we examine in detail the tomato colorless peel y mutant, which lacks the yellow flavonoid pigment naringenin chalcone, a major constituent of the fruit cuticle. Extensive transcript and metabolite profiling of this mutant revealed SlMYB12 as a key regulator in a transcription network that controls flavonoid accumulation in tomato peel. Moreover, the change in cuticle flavonoid composition enabled us to evaluate the importance of these constituents as barriers in the cuticle layer. Finally, because most commercial cultivars in the Far East are based on the y genetic background, discovery of the y gene will contribute also to tomato breeding programs.
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