Construction and Optimization of an Endometrial Injury Model in Mice by Transcervical Ethanol Perfusion
Construction and Optimization of an Endometrial Injury Model in Mice by Transcervical Ethanol Perfusion
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经宫颈乙醇灌注小鼠子宫内膜损伤模型的构建与优化
DOI：
10.1007/s43032-020-00296-2
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发表时间：
2020-09
影响因子：
2.9
通讯作者：
Lin Juntang
中科院分区：
文献类型：
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作者：
Zhang Shenghui;Sun Yuliang;Jiang Dongli;Chen Tongtong;Liu Ruihong;Li Xinyi;Lu Yilin;Qiao Liang;Pan Ying;Liu Yanli;Lin Juntang
本研究旨在通过微创方法建立稳定的宫内粘连（IUA）动物模型，以概括IUA的临床病理特征。将小鼠按乙醇处理时间随机分为各组（EtOH-10 s、EtOH-20 s、EtOH-40 s、EtOH-1 min、假手术组），间苯三酚放松子宫颈后，经子宫颈灌注95%乙醇诱导子宫内膜损伤。术后8天进行常规生化检查，评估肝肾功能；HE、Masson染色观察子宫形态及纤维化情况；免疫组化检测CD31和F4/80在子宫内膜中的表达。比较etoh - 40s组和假手术组小鼠的生育能力。正如预期的那样，乙醇处理时间与小鼠子宫损伤程度和肾功能损害程度呈正相关。特别是etoh - 40s组小鼠子宫内膜明显比假手术组小鼠薄，表现为严重坏死、腺体丢失、上皮及腺上皮细胞结构不完整、组织严重纤维化、炎症反应激活、胎数明显减少，符合重度IUA的临床特征。总之，本研究通过微创经宫颈乙醇灌注技术成功建立小鼠子宫内膜损伤模型，为IUA发病机制的深入研究提供支持，进一步促进IUA治疗方法的发展。
This study aimed to establish a stable animal model of intrauterine adhesion (IUA) using a minimally invasive method that recapitulates the clinicopathologic characteristics of IUA. Mice were randomly divided into groups based on the ethanol treatment time (the EtOH-10 s, EtOH-20 s, EtOH-40 s, EtOH-1 min, and sham operation groups), and after the cervix was relaxed with phloroglucinol, the uterine horn was perfused with 95% ethanol through the cervix to induce endometrial injury. Eight days after the procedure, routine biochemical assays were performed to assess liver and kidney function; HE and Masson staining were used to assess uterine morphology and fibrosis; and immunohistochemistry was performed to evaluate the expression of CD31 and F4/80 in the endometrium. Furthermore, the fertility of mice in the EtOH-40 s group and the sham operation group was compared. As expected, the ethanol treatment time was positively correlated with the degree of uterine damage and kidney dysfunction in mice. In particular, the endometria of mice in the EtOH-40 s group were significantly thinner than those of mice in the sham operation group and exhibited severe necrosis, glandular loss, incomplete epithelial and glandular epithelial cell structure, severe tissue fibrosis, an activated inflammatory response, and a significant decrease in the number of fetuses, consistent with the clinical characteristics of severe IUA. In conclusion, this study resulted in successful establishment, by a minimally invasive transcervical ethanol perfusion technique, of a mouse model of endometrial injury, which could support an in-depth study of IUA pathogenesis and further promote the development of IUA therapies.
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DOI：
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发表时间：
2004-09
期刊：
Zhonghua fu chan ke za zhi
影响因子：
--
作者：
Yan Hao;G. Zhai;A. Duan
通讯作者：
Yan Hao;G. Zhai;A. Duan
影响因子：
6.7
作者：
Hanstede, Miriam M. E.;van der Meij, Eva;Emanuel, Mark Hans
通讯作者：
Emanuel, Mark Hans
DOI：
10.1186/s12958-017-0234-9
发表时间：
2017-03-03
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Xiao L;Song Y;Huang W;Yang S;Fu J;Feng X;Zhou M
通讯作者：
Zhou M
影响因子：
18.2
作者：
Hamidzadeh K;Christensen SM;Dalby E;Chandrasekaran P;Mosser DM
通讯作者：
Mosser DM
影响因子：
9
作者：
Naghdi S;Slovinsky WS;Madesh M;Rubin E;Hajnóczky G
通讯作者：
Hajnóczky G