Myosin II dynamics are regulated by tension in intercalating cells.
Myosin II dynamics are regulated by tension in intercalating cells.
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DOI：
10.1016/j.devcel.2009.09.003
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发表时间：
2009-11
影响因子：
11.8
通讯作者：
Zallen JA
中科院分区：
文献类型：
--
作者：
Fernandez-Gonzalez R;Simoes Sde M;Röper JC;Eaton S;Zallen JA
果蝇的轴伸长是通过肌动球蛋白收缩能力驱动的极化细胞重排而发生的。肌球蛋白II通过收缩单个细胞边界促进邻近交换，而跨越多对细胞的肌球蛋白II结构的收缩导致玫瑰花环的形成。我们在这里展示了肌动球蛋白电缆在伸长过程中形成的频率比预期的要高，这表明电缆组装是一个活跃的过程。多细胞电缆是通过激光消融测量的机械张力增加的部位。光漂白后的荧光恢复实验表明，肌球蛋白II在张力增加的区域稳定在皮质。肌球蛋白II在异位力的作用下被招募，缓解张力会导致肌球蛋白的迅速丢失，这表明张力是皮质肌球蛋白定位的必要条件和充分条件。这些结果表明，肌球蛋白II的动力学受正反馈环中张力的调节，从而导致肌动球蛋白缆线的形成和有效的组织伸长。
Axis elongation in Drosophila occurs through polarized cell rearrangements driven by actomyosin contractility. Myosin II promotes neighbor exchange through the contraction of single cell boundaries, while the contraction of myosin II structures spanning multiple pairs of cells leads to rosette formation. Here we show that actomyosin cables form at a higher frequency than expected by chance during elongation, indicating that cable assembly is an active process. Multicellular cables are sites of increased mechanical tension as measured by laser ablation. Fluorescence recovery after photobleaching experiments show that myosin II is stabilized at the cortex in regions of increased tension. Myosin II is recruited in response to an ectopic force and relieving tension leads to a rapid loss of myosin, indicating that tension is necessary and sufficient for cortical myosin localization. These results demonstrate that myosin II dynamics are regulated by tension in a positive feedback loop that leads to actomyosin cable formation and efficient tissue elongation.
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