Anchorage‐independent growth of primary rat embryo cells is induced by platelet‐derived growth factor and inhibited by type‐beta transforming growth factor
Anchorage‐independent growth of primary rat embryo cells is induced by platelet‐derived growth factor and inhibited by type‐beta transforming growth factor
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原代大鼠胚胎细胞的贴壁非依赖性生长由血小板衍生生长因子诱导并被β型转化生长因子抑制
DOI：
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发表时间：
1986
影响因子：
5.6
通讯作者：
M. Sporn
中科院分区：
文献类型：
--
作者：
M. Anzano;A. Roberts;M. Sporn
研究人员测试了细胞转化过程中涉及的几种生长因子诱导原代大鼠胚胎（RE）细胞锚定不依赖（AI）生长的能力。我们的研究结果表明，在10%的小牛血清中，血小板衍生生长因子（PDGF）， 1-30 ng/ml，对Al生长的影响是所有生长因子中最强的。β型转化生长因子（TGF - β）本身不刺激Al的生长，它以剂量依赖性的方式抑制PDGF诱导的集落形成（ED50 ~ 0.03 ng/ml）。通过使用已建立的成纤维细胞系，NIH 3T3细胞，获得了定性相似的反应；原代细胞与培养细胞系的主要差异在于软琼脂培养中菌落的形成效率（在10 ng/ml PDGF存在下，菌落直径为1500 μm2的菌落生长效率分别为15%和90%）。由于Al生长已被证明与体内致瘤性密切相关，我们的研究结果表明，PDGF在适当反应细胞中的转化潜力不仅可以通过其自身受体的相互作用来控制，还可以通过TGF‐β等抑制因子的存在来控制。
Several growth factors implicated in the process of cellular transformation were tested for their ability to induce anchorage‐independent (AI) growth of primary rat embryo (RE) cells. Our results show that in the presence of 10% calf serum, platelet‐derived growth factor (PDGF), 1–30 ng/ml, has the strongest effect of all growth factors tested on Al growth. Type‐β transforming growth factor (TGF‐β), by itself, does not stimulate Al growth, and it inhibits the PDGF‐induced colony formation in a dose‐dependent manner (ED50 ∼ 0.03 ng/ml). Qualitatively similar responses are obtained by using an established line of fibroblasts, NIH 3T3 cells; the principal difference between the response of the primary cells and the established cell line is in colony‐forming efficiency in soft agar culture (15% and 90%, respectively, for growth of colonies > 1,500 μm2 diameter in the presence of 10 ng/ml PDGF). Since Al growth has been shown to correlate well with tumorigenicity in vivo, our results suggest that the transforming potential of PDGF in an appropriate responsive cell can be controlled not only through its interaction with its own receptor, but also by the presence of inhibitory factors such as TGF‐β.
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影响因子：
56.9
作者：
DOOLITTLE, RF;HUNKAPILLER, MW;ANTONIADES, HN
通讯作者：
ANTONIADES, HN
肾上皮细胞生长抑制剂的纯化。
DOI：
10.1073/pnas.77.10.5989
发表时间：
1980
影响因子：
11.1
作者：
Holley,RW;Böhlen,P;Fava,R;Baldwin,JH;Kleeman,G;Armour,R
通讯作者：
Armour,R
影响因子：
56.9
作者：
R. Tucker;G. Shipley;H. Moses;R. W. Holley
通讯作者：
R. Tucker;G. Shipley;H. Moses;R. W. Holley
e型转化生长因子的纯化。
影响因子：
4
作者：
Parnell,PG;Wunderlich,J;Carter,B;Halper,J
通讯作者：
Halper,J
影响因子：
3.8
作者：
Colleran, Gabrielle;McInerney, Niall;Sawyer, Elinor
通讯作者：
Sawyer, Elinor