Location of Tandem Repeats on Wheat Chromosome 5B and the Breakpoint on the 5BS Arm in Wheat Translocation T7BS.7BL-5BS Using Single-Copy FISH Analysis.
Location of Tandem Repeats on Wheat Chromosome 5B and the Breakpoint on the 5BS Arm in Wheat Translocation T7BS.7BL-5BS Using Single-Copy FISH Analysis.
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DOI：
10.3390/plants11182394
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发表时间：
2022-09-14
期刊：
影响因子：
--
通讯作者：
Fu S
中科院分区：
文献类型：
--
作者：
Zhang W;Tang Z;Luo J;Li G;Yang Z;Yang M;Yang E;Fu S
小麦（Triticum aestivum L.）富含串联重复序列，这有助于研究其核型进化。一些串联重复序列尚未组装到小麦基因组序列中。使用B2 DSC网络服务器中的blaetry工具进行的比对表明，5 B染色体的基因组序列（IWGSC RefSeq v2.1）不包含串联重复序列pTa-275，并且串联重复序列（GA）26分布在整个5 B染色体中。用pTa-275和（GA）26的寡核苷酸探针进行非变性荧光原位杂交（ND-FISH），结果表明，在中国春小麦5 B染色体上出现了pTa-275的一条信号带和（GA）26的两条信号带，表明这两种串联重复序列具有聚集分布的模式。单拷贝荧光原位杂交结果表明，pTa-275和（GA）26的聚簇区域分别位于~160-201 Mb、~153-157 Mb和~201-234 Mb之间。利用ND-FISH和单拷贝FISH技术，将广泛存在于西北欧小麦品种中的易位系T7BS.7BL-5 BS的5 BS部分的易位断裂点定位在157，749，421 bp ~ 158，555，080 bp之间（~0.8 Mb），该区域主要含有反转录转座子，确定了小麦5 B染色体上两种串联重复序列的聚类区域，为进一步研究提供了依据这条染色体的序列装配。本研究获得的易位系T7BS.7BL-5 BS上易位断裂点的序列特征有助于理解小麦染色体易位的机理。
Wheat (Triticum aestivum L.) is rich in tandem repeats, and this is helpful in studying its karyotypic evolution. Some tandem repeats have not been assembled into the wheat genome sequence. Alignment using the blastn tool in the B2DSC web server indicated that the genomic sequence of 5B chromosome (IWGSC RefSeq v2.1) does not contain the tandem repeat pTa-275, and the tandem repeat (GA)26 distributed throughout the whole 5B chromosome. The nondenaturing fluorescence in situ hybridization (ND-FISH) using the oligonucleotide (oligo) probes derived from pTa-275 and (GA)26 indicated that one signal band of pTa-275 and two signal bands of (GA)26 appeared on the 5B chromosome of Chinese Spring wheat, indicating the aggregative distribution patterns of the two kinds of tandem repeats. Single-copy FISH indicated that the clustering region of pTa-275 and the two clustering regions of (GA)26 were located in ~160–201 Mb, ~153–157 Mb, and ~201–234 Mb intervals, respectively. Using ND-FISH and single-copy FISH technologies, the translocation breakpoint on the 5BS portion of the translocation T7BS.7BL-5BS, which exists widely in north-western European wheat cultivars, was located in the region from 157,749,421 bp to 158,555,080 bp (~0.8 Mb), and this region mainly contains retrotransposons, and no gene was found. The clustering regions of two kinds of tandem repeats on wheat chromosome 5B were determined and this will be helpful to improve the future sequence assembly of this chromosome. The sequence characteristics of the translocation breakpoint on the translocation T7BS.7BL-5BS obtained in this study are helpful to understand the mechanism of wheat chromosome translocation.
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