CLP gene family, a new gene family of Cotesia vestalis bracovirus inhibits melanization of Plutella xylostella hemolymph
CLP gene family, a new gene family of Cotesia vestalis bracovirus inhibits melanization of Plutella xylostella hemolymph
复制标题
维氏小菜蛾病毒新基因家族CLP基因家族抑制小菜蛾血淋巴黑色化
DOI：
10.1111/1744-7917.12883
复制
发表时间：
2020-11
期刊：
影响因子：
4
通讯作者：
Chen Xue-Xin
中科院分区：
文献类型：
--
作者：
Wang Ze-Hua;Zhou Yue-Nan;Ye Xi-Qian;Wu Xiao-Tong;Yang Pei;Shi Min;Huang Jian-Hua;Chen Xue-Xin
多dna病毒（polydnavvirus, pdv）是寄生蜂的强制性共生体，在抑制宿主免疫防御中起着重要作用。虽然PDV基因抑制宿主黑化是已知的Microplitis bracovirus，但Cotesia bracovirus的功能同源物尚不清楚。本研究发现，vesttesia vestalis bracovirus （CvBV）可以抑制寄主小菜蛾（Plutella xylostella）幼虫寄生早期的血淋巴黑色素化，并且高表达CvBV基因的过表达会降低寄主酚氧化酶的活性。此外，CvBV‐7‐1在12小时内降低了宿主酚氧化酶活性，并且注射抗CvBV‐7‐1抗体增加了被寄生宿主幼虫的黑化。进一步分析表明，CvBV‐7‐1和来自其他Cotesia小链病毒的三个同源物具有C末端亮氨酸/异亮氨酸丰富区域，并且在抑制黑色素化方面具有相似的功能。因此，我们提出了一个新的bracovirus基因家族，并将其命名为C -末端亮氨酸/异亮氨酸富蛋白（CLP）。果蝇血细胞中CvBV‐7‐1的异位表达增加了细菌抑制黑素化的易感性，并减少了被寄生蜂布氏瘦霉寄生的黑素囊化。当CvBV‐7‐1基因的功能被阻断时，蜂蛹的形成率和蜂蛹的羽化率均受到影响。我们的研究结果表明，来自Cotesia bracovirus的CLP基因编码含有C末端亮氨酸/异亮氨酸丰富区域的蛋白质，并在寄生早期起到黑化抑制作用，这对成功寄生至关重要。
Polydnaviruses (PDVs) are obligatory symbionts of parasitoid wasps and play an important role in suppressing host immune defenses. Although PDV genes that inhibit host melanization are known in Microplitis bracovirus, the functional homologs in Cotesia bracoviruses remain unknown. Here, we find that Cotesia vestalis bracovirus (CvBV) can inhibit hemolymph melanization of its host, Plutella xylostella larvae, during the early stages of parasitization, and that overexpression of highly expressed CvBV genes reduced host phenoloxidase activity. Furthermore, CvBV‐7‐1 in particular reduced host phenoloxidase activity within 12 h, and the injection of anti‐CvBV‐7‐1 antibody increased the melanization of parasitized host larvae. Further analyses showed that CvBV‐7‐1 and three homologs from other Cotesia bracoviruses possessed a C‐terminal leucine/isoleucine‐rich region and had a similar function in inhibiting melanization. Therefore, a new family of bracovirus genes was proposed and named as C‐terminal Leucine/isoleucine‐rich Protein (CLP). Ectopic expression of CvBV‐7‐1 in Drosophila hemocytes increased susceptibility to bacterial repression of melanization and reduced the melanotic encapsulation of parasitized D. melanogaster by the parasitoid Leptopilina boulardi. The formation rate of wasp pupae and the eclosion rate of C. vestalis were affected when the function of CvBV‐7‐1 was blocked. Our findings suggest that CLP genes from Cotesia bracoviruses encoded proteins that contain a C‐terminal leucine/isoleucine‐rich region and function as melanization inhibitors during the early stage of parasitization, which is important for successful parasitization.
登录
查看更多内容
影响因子：
12.3
作者：
Desjardins, Christopher A.;Gundersen-Rindal, Dawn E.;Hostetler, Jessica B.;Tallon, Luke J.;Fadrosh, Douglas W.;Fuester, Roger W.;Pedroni, Monica J.;Haas, Brian J.;Schatz, Michael C.;Jones, Kristine M.;Crabtree, Jonathan;Forberger, Heather;Nene, Vishvanath
通讯作者：
Nene, Vishvanath
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.8
作者：
Lu, Zhiqiang;Beck, Markus H.;Strand, Michael R.
通讯作者：
Strand, Michael R.
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
3.8
作者：
Bitra, Kavita;Zhang, Shu;Strand, Michael R.
通讯作者：
Strand, Michael R.