RNA processing is a limiting step for murine tumor necrosis factor beta expression in response to interleukin-2-猫眼课题宝

RNA 加工是小鼠肿瘤坏死因子 β 表达响应白细胞介素 2 的限制步骤

DOI：

10.1128/mcb.10.11.5865-5875.1990

发表时间：

1990

期刊：

Molecular and Cellular Biology

影响因子：

5.3

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

D. Weil;S. Brosset;F. Dautry

我们以前曾报道，肿瘤坏死因子β（TNF β）的表达诱导白细胞介素-2（IL-2）在小鼠淋巴细胞T细胞系CTLL-2。在这项研究中，我们的特点是细胞核和细胞质TNF β转录，并评估其在TNF β基因表达的作用。TNF β表达的一个独特特征是核前体的积累，这反映了缓慢的核RNA加工。因此，在IL-2转录诱导TNF β后，细胞质信使的出现延迟。我们还发现，两个信使，完全剪接的信使和内含子3保留的信使，被输出到细胞质和积极翻译。在伴刀豆球蛋白A刺激的脾细胞中观察到相同的表达模式，尽管表达水平远低于CTLL-2细胞。简单的遗传结构和核前体的高水平积累使TNF β成为一个特别有吸引力的模型系统，用于研究RNA加工和部分剪接信使的细胞质转运。

We have previously reported that tumor necrosis factor beta (TNF beta) expression is induced by interleukin-2 (IL-2) in the murine lymphocytic T-cell line CTLL-2. In this study, we have characterized the nuclear and cytoplasmic TNF beta transcript and assessed their role in TNF beta gene expression. A unique feature of TNF beta expression was the accumulation of nuclear precursors, which reflected a slow nuclear RNA processing. As a consequence, there was a delay in the appearance of cytoplasmic messengers after the transcriptional induction of TNF beta by IL-2. We also found that two messengers, the fully spliced messenger and an intron 3-retaining messenger, were exported to the cytoplasm and actively translated. The same pattern of expression was observed in concanavalin A-stimulated splenocytes, although the level of expression was much lower than in CTLL-2 cells. The simple genetic structure and the high level of accumulation of nuclear precursors make TNF beta a particularly attractive model system to use for studies of RNA processing and cytoplasmic transport of partially spliced messengers.