Neonatal alcohol exposure disrupts hippocampal neurogenesis and contextual fear conditioning in adult rats.
Neonatal alcohol exposure disrupts hippocampal neurogenesis and contextual fear conditioning in adult rats.
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DOI：
10.1016/j.brainres.2011.07.027
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发表时间：
2011-09-15
期刊：
影响因子：
2.9
通讯作者：
Klintsova AY
中科院分区：
文献类型：
--
作者：
Hamilton GF;Murawski NJ;St Cyr SA;Jablonski SA;Schiffino FL;Stanton ME;Klintsova AY
发育中的酒精暴露可以永久改变大脑结构，并在行为的许多方面产生功能障碍，包括学习和记忆。本研究评估了新生儿酒精暴露对海马齿状回成年神经发生的影响，以及这种暴露对海马依赖性情境恐惧条件反射的影响。暴露于酒精的大鼠（AE）在出生后第4-9天（人类妊娠晚期）接受5.25 g/kg/天的酒精，以类似狂欢的方式。包括两个对照组：假插管组（SI）和吸吮对照组（SC）。断奶后，将动物圈养在群居笼中（3只/笼）。在PD 80，动物注射200 mg/kg BrdU。两小时后处死一半动物。其余的在PD 114处死以评价细胞存活;测试未注射BrdU的单独AE、SI和SC大鼠的背景暴露前易化效应（CPFE; PD 117）。在PD 80时，AE、SI和SC组中BrdU+细胞数量无差异。在PD 114，AE大鼠的细胞存活率显著降低，表明发育中的酒精暴露损害了新细胞融入网络和存活的能力。行为测试的SC和SI组preexposed到培训上下文24小时之前，接受1.5mA的2s footshock冻结显着超过他们的同行preexposed到一个替代的上下文测试过程中。AE大鼠不能显示CPFE。目前的研究表明，有害的，长期的影响，发展酒精暴露海马成人神经发生和上下文恐惧条件反射。
Developmental alcohol exposure can permanently alter brain structures and produce functional impairments in many aspects of behavior, including learning and memory. This study evaluates the effect of neonatal alcohol exposure on adult neurogenesis in the dentate gyrus of the hippocampus and the implications of such exposure for hippocampus-dependent contextual fear conditioning. Alcohol-exposed rats (AE) received 5.25 g/kg/day of alcohol on postnatal days (PD) 4-9 (third trimester in humans), in a binge-like manner. Two control groups were included: sham-intubated (SI) and suckle-control (SC). Animals were housed in social cages (3/cage) after weaning. On PD80, animals were injected with 200 mg/kg BrdU. Half of the animals were sacrificed two hours later. The remainder were sacrificed on PD114 to evaluate cell survival; separate AE, SI, and SC rats not injected with BrdU were tested for the context preexposure facilitation effect (CPFE; ∼PD117). There was no difference in the number of BrdU+ cells in AE, SI and SC groups on PD80. On PD114, cell survival was significantly decreased in AE rats, demonstrating that developmental alcohol exposure damages new cells' ability to incorporate into the network and survive. Behaviorally tested SC and SI groups preexposed to the training context 24h prior to receiving a 1.5mA 2s footshock froze significantly more during the context test than their counterparts preexposed to an alternate context. AE rats failed to show the CPFE. The current study shows the detrimental, long-lasting effects of developmental alcohol exposure on hippocampal adult neurogenesis and contextual fear conditioning.
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影响因子：
9.8
作者：
Dupret D;Fabre A;Döbrössy MD;Panatier A;Rodríguez JJ;Lamarque S;Lemaire V;Oliet SH;Piazza PV;Abrous DN
通讯作者：
Abrous DN
影响因子：
2.9
作者：
Gil-Mohapel, Joana;Boehme, Fanny;Christie, Brian R.
通讯作者：
Christie, Brian R.
影响因子：
3.5
作者：
Clark, Peter J.;Brzezinska, Weronika J.;Puchalski, Emily K.;Krone, David A.;Rhodes, Justin S.
通讯作者：
Rhodes, Justin S.
DOI：
10.1111/j.1530-0277.2001.tb02129.x
发表时间：
2001-01-01
影响因子：
3.2
作者：
Bonthius, DJ;Woodhouse, J;Lothman, EW
通讯作者：
Lothman, EW
影响因子：
2.7
作者：
GOODLETT, CR;PETERSON, SD
通讯作者：
PETERSON, SD