Centromere autoantigens are associated with the nucleolus.
Centromere autoantigens are associated with the nucleolus.
复制标题
着丝粒自身抗原与核仁相关。
DOI：
10.1016/0014-4827(92)90181-7
复制
发表时间：
1992
影响因子：
3.7
通讯作者：
Press,RI
中科院分区：
文献类型：
--
作者：
Ochs,RL;Press,RI
由于它们在硬皮病中作为靶抗原的重要性，以及硬皮病中的所有其他主要自身抗原都可以定位于间期核仁，我们有兴趣进一步研究间期着丝粒与核仁之间的潜在关系。用来自脊型硬皮病患者的人类抗着丝粒自身抗体(ACA)作为间接免疫荧光显微镜的探针，我们观察到着丝粒在非随机的间期“聚集”，其分布类似于核仁。通过双标记免疫荧光比较着丝粒与核仁蛋白Ki-67、纤维蛋白或蛋白质B23(核磷蛋白)的定位，间期着丝粒似乎定位在核仁周围和核仁内。一些不同的ACA血清在Hep-2、HeLa、PtK2、印度芒果、3T3和NRK细胞上进行了测试，所有结果都表明着丝粒和核仁之间存在共定位。免疫电子显微镜显示，间期着丝粒自由分布于核质内，与核膜接触，与核仁周缘接触，并完全嵌入核仁的范围内。有趣的是，放线菌素D处理使着丝粒从分离的核仁中分离出来。为了确定间期着丝粒是否是核仁的组成部分，按照经典方法分离核仁。通过双标记免疫荧光、免疫电子显微镜和Western blotting，证明着丝粒自身抗原与分离的核仁结合。这些研究证明，一些间期着丝粒可以与间期核仁相关，甚至可以被认为是间期核仁的一部分。此外，硬皮病中的所有主要自身抗原现在都可以定位于核仁。
Because of their importance as target antigens in scleroderma and since all other major autoantigens in scleroderma can be localized to the interphase nucleolus, we were interested in a further investigation of the potential relationship between interphase centromeres and the nucleolus. Using human anticentromere autoantibodies (ACA) from patients with the CREST form of scleroderma as probes in indirect immunofluorescence microscopy, we observed nonrandom interphase “clumping” of centromeres in a distribution suggestive of nucleoli. By double-label immunofluorescence comparing the localization of centromeres to nucleolar proteins Ki-67, fibrillarin, or protein B23 (nucleophosmin), interphase centromeres appeared to be localized around and within nucleoli. A number of different ACA sera were tested on HEp-2, HeLa, PtK2, Indian muntjac, 3T3, and NRK cells, all with identical results indicating colocalization between centromeres and nucleoli. Immunoelectron microscopy revealed that interphase centromeres were distributed free in the nucleoplasm, in contact with the nuclear envelope, in contact with and on the periphery of nucleoli, and totally embedded within the confines of the nucleolus itself. Interestingly, actinomycin D treatment dissociated centromeres from localization within the segregated nucleolus. To determine if interphase centromeres were integral components of nucleoli, nucleoli were isolated according to classical methods. By double-label immunofluorescence, immunoelectron microscopy, and Western blotting, it was demonstrated that centromere autoantigens copurified with isolated nucleoli. These studies offer proof that some interphase centromeres can be associated with, and may even be considered part of, the interphase nucleolus. Furthermore, all of the major autoantigens in scleroderma can now be localized to the nucleolus.
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使用人自身免疫血清表征核仁蛋白作为自身抗原。
影响因子：
27.4
作者：
J. Pfeifle;F. Anderer;M. Franke
通讯作者：
M. Franke
通过免疫细胞化学和电子显微镜水平的原位杂交对核仁染色质进行定位。
DOI：
--
发表时间：
1991
期刊：
Electron Microscopy Reviews
影响因子：
--
作者：
M. Thiry;Ulrich Scheer;Guy Goessens
通讯作者：
Guy Goessens
神经元间期核中动粒蛋白和卫星 DNA 的排列：γ-氨基丁酸 (GABA) 诱导的变化。
影响因子：
3.7
作者：
T. Holowacz;U. De Boni
通讯作者：
U. De Boni
影响因子：
3.7
作者：
HADLACZKY, G;WENT, M;RINGERTZ, NR
通讯作者：
RINGERTZ, NR
小鼠支持细胞中的核仁转录活性取决于着丝粒排列。
影响因子：
3.7
作者：
T. Haaf;C. Steinlein;M. Schmid
通讯作者：
M. Schmid