Characteristics of the bacterial microbiome in association with common intestinal parasites in irritable bowel syndrome.
Characteristics of the bacterial microbiome in association with common intestinal parasites in irritable bowel syndrome.
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DOI：
10.1038/s41424-018-0027-2
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发表时间：
2018-06-19
影响因子：
3.6
通讯作者：
Bytzer P
中科院分区：
文献类型：
--
作者：
Krogsgaard LR;Andersen LO';Johannesen TB;Engsbro AL;Stensvold CR;Nielsen HV;Bytzer P
在肠易激综合征（IBS）患者中，肠道寄生虫的患病率较低，但与细菌微生物组改变的潜在关联在很大程度上仍未被探索。我们的目的是调查IBS患者体内寄生虫和细菌之间的关系，以确定潜在的跨界微生物特征。从丹麦背景人群中收集粪便样本，根据罗马III IBS标准分为IBS（n = 119）、非特异性胃肠道（GI）症状（n = 114）和无症状对照（n = 186）。对细菌（16 S）和真核生物（18 S）核糖体DNA进行测序，并将18 S数据与常规寄生虫实验室试验的数据合并。根据症状组和寄生虫定植状态分析细菌微生物组。IBS和对照组的细菌丰富度和多样性相似，但在非特异性胃肠道症状的患者中更高。与对照组相比，在患有IBS和非特异性GI症状的个体中检测到较高丰度的拟杆菌和较低丰度的粪杆菌。主成分分析表明，寄生虫定植，而不是症状组相关的细菌组成的差异。在IBS组（39%）和以拟杆菌属为主的样本中检测到的寄生虫频率最低。较高的细菌丰富度和多样性被发现在寄生虫阳性样品从控制和那些与非特异性胃肠道症状，但没有在个人与IBS。寄生虫定植，而不是细菌组成，IBS和健康对照组之间的个人不同。寄生虫定植与丰富多样的细菌微生物组相关;然而，这种关联在IBS中发生了改变。
A low prevalence of intestinal parasites has been identified in individuals with irritable bowel syndrome (IBS), but potential associations with alterations in the bacterial microbiome remain largely unexplored. We aimed to investigate the relationship between parasites and bacteria in individuals with IBS in order to identify potential trans-kingdom microbial characteristics. Stool samples were collected from the Danish background population classified into IBS (n = 119), unspecific gastrointestinal (GI) symptoms (n = 114), and asymptomatic controls (n = 186) based on the Rome III criteria for IBS. Bacterial (16S) and eukaryotic (18S) ribosomal DNA was sequenced, and 18S data were merged with data from conventional parasite laboratory tests. The bacterial microbiome was analyzed according to symptom group and parasite colonization status. Bacterial richness and diversity were similar for IBS and controls but higher in those with unspecific GI symptoms. A higher abundance of Bacteroides and a lower abundance of Faecalibacterium were detected in individuals with IBS and unspecific GI symptoms compared with controls. Principal component analyses indicated differences in bacterial composition related to parasite colonization rather than symptom group. Parasites were detected at the lowest frequency in the IBS group (39%) and in samples dominated by Bacteroides. Higher bacterial richness and diversity were found in parasite-positive samples from controls and those with unspecific GI symptoms but not in individuals with IBS. Parasite colonization, rather than bacterial composition, differed between individuals with IBS and healthy controls. Parasite colonization was associated to a rich and diverse bacterial microbiome; however, this association was altered in IBS.
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影响因子：
15.5
作者：
Nagel R;Traub RJ;Allcock RJ;Kwan MM;Bielefeldt-Ohmann H
通讯作者：
Bielefeldt-Ohmann H
影响因子：
4.6
作者：
Choo JM;Leong LE;Rogers GB
通讯作者：
Rogers GB
影响因子：
9.4
作者：
Jokelainen, Pikka;Jensen, Betina Hebbelstrup;Stensvold, Christen R.
通讯作者：
Stensvold, Christen R.
影响因子：
24.5
作者：
Jalanka-Tuovinen, Jonna;Salojarvi, Jarkko;de Vos, Willem M.
通讯作者：
de Vos, Willem M.
影响因子：
4.2
作者：
Andersen, Lee O'Brien;Bonde, Ida;Stensvold, Christen Rune
通讯作者：
Stensvold, Christen Rune