Ancient vertebrate dermal armor evolved from trunk neural crest.
Ancient vertebrate dermal armor evolved from trunk neural crest.
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DOI：
10.1073/pnas.2221120120
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发表时间：
2023-07-25
影响因子：
11.1
通讯作者：
Bronner, Marianne E.
中科院分区：
文献类型：
--
作者：
Stundl, Jan;Martik, Megan L.;Chen, Donglei;Raja, Desingu Ayyappa;Franek, Roman;Pospisilova, Anna;Psenicka, Martin;Metscher, Brian D.;Braasch, Ingo;Haitina, Tatjana;Cerny, Robert;Ahlberg, Per E.;Bronner, Marianne E.
早期脊椎动物的身体从头到尾都覆盖着广泛的真皮盔甲，由牙本质和骨骼组成。长期以来，对于这种盔甲是来自神经脊、中胚层，还是两者兼而有之，一直存在争议。由于成牙本质细胞(牙本质产生细胞)完全来源于神经脊，我们探索了胸鳍鱼类早期分支谱系--胸鱼真皮盔甲的骨骼成分的发育起源。在这里，我们展示了胸骨的躯干神经脊产生成骨细胞，产生鳞片的骨。总之，我们的结果支持神经脊沿整个身体轴线的原始骨骼形成作用，后来在脊椎动物进化过程中逐渐限制在颅骨区域。骨骼是脊椎动物在进化上的新奇事物，很可能最初是作为祖先真皮盔甲的一部分出现的，真皮盔甲由成骨和成牙成分组成。这些早期脊椎动物的结构是由中胚层细胞还是神经脊细胞产生的，一直是一个相当有争议的问题。为了研究真皮盔甲的骨性部分的发育起源，我们在小鲟鱼身上进行了体内血统追踪，这是一种保留了广泛的颅后真皮骨骼的非硬骨鱼的代表。结果明确地表明，胸骨干神经脊细胞可分化为成骨细胞。转录图谱进一步揭示了小腿鳞片和Bichir鳞片中神经脊基因的特征。最后，对鳍鱼真皮盔甲的组织学和显微CT分析表明，它们的鳞片和鳞片是由骨、牙本质和高矿化的覆盖组织以不同的组合形成的，与第一代装甲脊椎动物相似。综上所述，我们的结果支持神经脊沿整个身体轴线的原始骨骼形成作用，后来在脊椎动物进化过程中逐渐限制在颅骨区域。因此，神经脊是一项关键的进化创新，推动了真皮盔甲沿着整个身体轴线的起源和多样化。
The body of early vertebrates was covered from the head to tail with extensive dermal armor, comprised of dentin and bone. There has long been controversy over whether this armor arose from the neural crest, mesoderm, or both. Since odontoblasts (dentin-producing cells) are exclusively neural crest derived, we probed the developmental origin of the bony component of the dermal armor in the sterlet sturgeon, an early branching lineage of ray-finned fishes. Here, we show that trunk neural crest of the sterlet gives rise to osteoblasts, producing the bone of the scutes. Together, our results support a primitive skeletogenic role for the neural crest along the entire body axis, that was later progressively restricted to the cranial region during vertebrate evolution. Bone is an evolutionary novelty of vertebrates, likely to have first emerged as part of ancestral dermal armor that consisted of osteogenic and odontogenic components. Whether these early vertebrate structures arose from mesoderm or neural crest cells has been a matter of considerable debate. To examine the developmental origin of the bony part of the dermal armor, we have performed in vivo lineage tracing in the sterlet sturgeon, a representative of nonteleost ray-finned fish that has retained an extensive postcranial dermal skeleton. The results definitively show that sterlet trunk neural crest cells give rise to osteoblasts of the scutes. Transcriptional profiling further reveals neural crest gene signature in sterlet scutes as well as bichir scales. Finally, histological and microCT analyses of ray-finned fish dermal armor show that their scales and scutes are formed by bone, dentin, and hypermineralized covering tissues, in various combinations, that resemble those of the first armored vertebrates. Taken together, our results support a primitive skeletogenic role for the neural crest along the entire body axis, that was later progressively restricted to the cranial region during vertebrate evolution. Thus, the neural crest was a crucial evolutionary innovation driving the origin and diversification of dermal armor along the entire body axis.
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影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
0.9
作者：
Connolly, M. H.;Yelick, P. C.
通讯作者：
Yelick, P. C.
DOI：
10.1073/pnas.1713827114
发表时间：
2017-12-12
影响因子：
11.1
作者：
Gillis, J. Andrew;Alsema, Els C.;Criswell, Katharine E.
通讯作者：
Criswell, Katharine E.
影响因子：
2.5
作者：
Cebra-Thomas, Judith A.;Terrell, Anne;Gilbert, Scott F.
通讯作者：
Gilbert, Scott F.