Intact long-type dupA as a marker for gastroduodenal diseases in Okinawan subpopulation, Japan.
Intact long-type dupA as a marker for gastroduodenal diseases in Okinawan subpopulation, Japan.
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DOI：
10.1111/j.1523-5378.2012.00994.x
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发表时间：
2013-02
期刊：
影响因子：
4.4
通讯作者：
Yamaoka Y
中科院分区：
文献类型：
--
作者：
Takahashi A;Shiota S;Matsunari O;Watada M;Suzuki R;Nakachi S;Kinjo N;Kinjo F;Yamaoka Y
幽门螺杆菌dupA可根据突变的存在与否分为两种类型。此外，全序列数据显示，dupA具有两种不同长度的类型，这取决于推定的5'区域中约600 bp的存在（存在;长型和不存在;短型），这在以前的研究中没有考虑到。从日本南部岛屿冲绳分离的319株菌株被包括在内。用聚合酶链反应检测dupA和cagA的状态。通过DNA测序确定长型dupA中突变的存在。长型dupA的患病率为26.3%（84/319）。序列分析表明，84株长型dupA中仅有6株（7.1%）发生了终止密码子点突变。有趣的是，没有移码突变的完整长型dupA，而不是短型dupA与胃溃疡和胃癌显著相关，而不是胃炎（P = 0.001和P = 0.019，分别）。经年龄、性别和cagA校正后，与胃炎相比，完整长型dupA的存在与胃溃疡和胃癌显著相关（比值比[OR] = 3.35，95%置信区间[CI] = 1.55-7.24和OR = 4.14，95% CI = 1.23-13.94）。完整的长型dupA是日本冲绳严重后果的真实的毒力标志物。以前从基于PCR的方法中获得的信息（未考虑长型dupA）必须谨慎解释。
Helicobacter pylori dupA can be divided into two types according to the presence or absence of the mutation. In addition, full-sequenced data revealed that dupA has two types with different lengths depend on the presence of approximately 600 bp in the putative 5' region (presence; long-type and absence; short-type), which has not been taken into account in previous studies. A total of 319 strains isolated from Okinawa, the south islands of Japan, were included. The status of dupA and cagA was determined by polymerase chain reaction. The presence of mutations in long-type dupA was determined by DNA sequencing. The prevalence of long-type dupA was 26.3% (84/319). Sequence analysis showed that there were only 6 cases (7.1%) with point mutations lead to stop codon among 84 long-type dupA strains studied. Interestingly, intact long-type dupA without frameshift mutation, but not short-type dupA was significantly associated with gastric ulcer and gastric cancer than gastritis (P = 0.001 and P = 0.019, respectively). After adjustment by age, gender and cagA, the presence of intact long-type dupA was significantly associated with gastric ulcer and gastric cancer compared with gastritis (odds ratio [OR] = 3.35, 95% confidence interval [CI] = 1.55–7.24 and OR = 4.14, 95% CI = 1.23–13.94, respectively). Intact long-type dupA is a real virulence marker for severe outcomes in Okinawa, Japan. The previous information gained from PCR-based methods without taking long-type dupA into account must be interpreted with caution.
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影响因子：
6.4
作者：
Hussein, Nawfal R.;Argent, Richard H.;Atherton, John C.
通讯作者：
Atherton, John C.
影响因子：
7.6
作者：
Nobuta, A;Asaka, M;Shinomura, J
通讯作者：
Shinomura, J
影响因子：
4.2
作者：
Shiota S;Matsunari O;Watada M;Hanada K;Yamaoka Y
通讯作者：
Yamaoka Y
DOI：
10.1073/pnas.96.22.12778
发表时间：
1999-10-26
影响因子：
11.1
作者：
Gerhard, M;Lehn, N;Prinz, C
通讯作者：
Prinz, C
影响因子：
14.2
作者：
Nguyen, L. T.;Uchida, T.;Moriyama, M.
通讯作者：
Moriyama, M.