Analysis of the expression of cancer-associated fibroblast- and EMT-related proteins in submucosal invasive colorectal cancer.
Analysis of the expression of cancer-associated fibroblast- and EMT-related proteins in submucosal invasive colorectal cancer.
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粘膜下浸润性结直肠癌中癌相关成纤维细胞和emt相关蛋白的表达分析。
DOI：
10.7150/jca.25646
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发表时间：
2018
影响因子：
3.9
通讯作者：
Matsumoto T
中科院分区：
文献类型：
--
作者：
Sugai T;Uesugi N;Kitada Y;Yamada N;Osakabe M;Eizuka M;Sugimoto R;Fujita Y;Kawasaki K;Yamamoto E;Yamano H;Suzuki H;Matsumoto T
目的：近年来的研究表明，肿瘤相关成纤维细胞(CAF)和上皮-间充质转化(EMT)在结直肠癌的发生、发展和转移中起重要作用。尽管预测粘膜下浸润性结直肠癌(SiCRC)的淋巴转移很重要，但CAF和EMT与SiCRC淋巴转移的关系尚未得到研究。本研究旨在分析CaF和EMT相关蛋白在SiCRC中的表达模式。材料和方法：采用免疫组织化学方法检测29例有淋巴结转移和80例无淋巴结转移的肝癌组织中α-平滑肌肌动蛋白、CD10、泊多普宁、成纤维细胞特异性蛋白1、脂肪细胞增强子结合蛋白1、肌动蛋白相关蛋白[锌指蛋白SNAI2、扭转相关蛋白1]的表达。我们使用层次聚类分析研究了生物标记物的表达模式。因此，根据CAF和EMT相关标记物的表达模式以及这些亚组与临床病理变量的关系，我们建立了四个亚组。结果：在多因素分析中，亚组2以所有标志物的高表达为特征，与淋巴结转移相关(p<；0.01)。接下来，我们研究了单个生物标记物与淋巴转移的关系。多因素分析显示，中分化腺癌与淋巴结转移显著相关(p<；0.05)。结论：我们的研究结果表明，CAF标志物和EMT相关蛋白的表达模式可能允许将患者分层为SiCRC中淋巴结转移的危险类别。
Objective: Recent studies have shown that cancer-associated fibroblasts (CAFs) and the epithelial-mesenchymal transition (EMT) play important roles in the progression and metastasis of CRC. Although prediction of lymph node metastasis in submucosal invasive colorectal cancer (SiCRC) is important, the relationships of CAF and EMT with lymph node metastasis of SiCRC have not yet been examined. Here, we aimed to analyze the expression patterns of CAF- and EMT-related proteins in SiCRC. Materials and Methods: The expression of CAF-related markers, including α-smooth muscle actin, CD10, podoplanin, fibroblast specific protein 1, and adipocyte enhancer-binding protein 1, and EMT-related proteins [zinc finger protein SNAI2 (ZEB1) and twist-related protein 1 (TWIST1) in SiCRC with (n = 29) or without (n = 80) lymph node metastasis was examined by immunohistochemistry. We examined the expression patterns of biomarkers using hierarchical cluster analysis. Consequently, four subgroups were established based on the expression patterns of CAF- and EMT-related markers, and the associations of these subgroups with clinicopathological variables. Results: In multivariate analysis, subgroup 2, which was characterized by high expression of all markers, was correlated with lymph node metastasis (p < 0.01). Next, we examined the associations of individual biomarkers with lymph node metastasis. Multivariate analysis showed that moderately differentiated adenocarcinoma was significantly associated with lymph node metastasis (p < 0.05). Conclusions: Our findings showed that expression patterns of CAF markers and EMT-related proteins may allow for stratification of patients into risk categories for lymph node metastasis in SiCRC.
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hTERT/ZEB1 复合物直接调节 E-钙粘蛋白促进结直肠癌上皮间质转化 (EMT)
影响因子：
--
作者：
Qin Y;Tang B;Hu CJ;Xiao YF;Xie R;Yong X;Wu YY;Dong H;Yang SM
通讯作者：
Yang SM
影响因子：
5.2
作者：
Conti J;Thomas G
通讯作者：
Thomas G
影响因子：
5.2
作者：
Marzouk O;Schofield J
通讯作者：
Schofield J
影响因子：
4.5
作者：
Choi SY;Sung R;Lee SJ;Lee TG;Kim N;Yoon SM;Lee EJ;Chae HB;Youn SJ;Park SM
通讯作者：
Park SM
影响因子：
24.5
作者：
MORSON, BC;WHITEWAY, JE;WILLIAMS, CB
通讯作者：
WILLIAMS, CB