Sub-inhibitory concentrations of trans-cinnamaldehyde attenuate virulence in Cronobacter sakazakii in vitro.
Sub-inhibitory concentrations of trans-cinnamaldehyde attenuate virulence in Cronobacter sakazakii in vitro.
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DOI：
10.3390/ijms15058639
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发表时间：
2014-05-15
影响因子：
5.6
通讯作者：
Venkitanarayanan K
中科院分区：
文献类型：
--
作者：
Amalaradjou MA;Kim KS;Venkitanarayanan K
阪崎慢杆菌是一种食源性病原体，可引起新生儿和儿童的一种危及生命的脑膜炎、坏死性结肠炎和脑膜脑炎。流行病学研究表明，婴儿配方奶粉是阪崎肠杆菌的主要来源。在这项研究中，我们通过细胞培养、显微镜观察和基因表达分析，研究了肉桂中的一种成分反式肉桂醛(TC)的亚抑制浓度(SIC)对体外降低阪崎肠杆菌毒力的效果。TC显著抑制阪崎肠杆菌对人和大鼠肠上皮细胞、人脑微血管内皮细胞的黏附和侵袭(p≤0.05)。此外，TC还能抑制阪崎肠杆菌在人巨噬细胞中的存活和复制。我们还观察到，TC通过抑制一氧化氮的产生，降低了阪崎肠杆菌引起大鼠肠道细胞死亡的能力。基因表达研究结果表明，TC显著下调了阪崎肠杆菌中与运动、宿主组织黏附和侵袭、巨噬细胞存活和内毒素合成有关的毒力基因。TC在削弱这些主要毒力因子方面的有效性凸显了它在预防和/或控制由这种病原体引起的感染方面的潜在用途。
Cronobacter sakazakii is a foodborne pathogen, which causes a life-threatening form of meningitis, necrotizing colitis and meningoencephalitis in neonates and children. Epidemiological studies implicate dried infant formula as the principal source of C. sakazakii. In this study, we investigated the efficacy of sub-inhibitory concentrations (SIC) of trans-cinnamaldehyde (TC), an ingredient in cinnamon, for reducing C. sakazakii virulence in vitro using cell culture, microscopy and gene expression assays. TC significantly (p ≤ 0.05) suppressed C. sakazakii adhesion to and invasion of human and rat intestinal epithelial cells, and human brain microvascular endothelial cells. In addition, TC inhibited C. sakazakii survival and replication in human macrophages. We also observed that TC reduced the ability of C. sakazakii to cause cell death in rat intestinal cells, by inhibiting nitric oxide production. Results from gene expression studies revealed that TC significantly downregulated the virulence genes critical for motility, host tissue adhesion and invasion, macrophage survival, and LPS (Lipopolysaccharide) synthesis in C. sakazakii. The efficacy of TC in attenuating these major virulence factors in C. sakazakii underscores its potential use in the prevention and/or control of infection caused by this pathogen.
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