Pyriproxyfen is metabolized by P450s associated with pyrethroid resistance in An. gambiae.
Pyriproxyfen is metabolized by P450s associated with pyrethroid resistance in An. gambiae.
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羟基芬芬被An中的P450代谢。冈比亚。
DOI：
10.1016/j.ibmb.2016.09.001
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发表时间：
2016-11
影响因子：
3.8
通讯作者：
Paine MJ
中科院分区：
文献类型：
--
作者：
Yunta C;Grisales N;Nász S;Hemmings K;Pignatelli P;Voice M;Ranson H;Paine MJ
对拟除虫菊酯的抗药性在疟疾病媒冈比亚按蚊中广泛存在，这导致人们对以拟除虫菊酯为唯一活性成分的蚊帐的未来效力感到担忧。目前正在非洲试验将一种保幼激素类似物吡丙醚掺入经拟除虫菊酯处理的蚊帐。拟除虫菊酯抗性通常与P450表达水平升高相关，包括CYP 6 M2、6P 2、6P 3、6P 4、6P 5、6 Z2和9 J5。在大肠杆菌中表达这些P450后，我们发现它们都能够代谢PPF。还检查了氯菊酯、溴氰菊酯和PPF对这些P450的抑制作用。溴氰菊酯和氯菊酯是除CYP 6 Z2（IC 50> 10 μ M）外所有P450的二乙氧基荧光素（DEF）活性的中度抑制剂（IC 50 1-10 μ M），而PPF对除CYP 6 Z2和6P 2（IC 50 1-10 μ M）外的所有P450（IC 50> 10 μ M）显示较弱的抑制作用。我们发现PPF和其他杀虫剂类之间的交叉抗性水平较低的证据，通过比较PPF在抑制变态和诱导雌性不育的杀虫剂敏感菌株的An。gambiae和来自科特迪瓦的多重抗性菌株。吡丙醚通过与拟除虫菊酯抗性相关的P450代谢。拟除虫菊酯可协同吡丙醚活性。吡丙醚的功效在多重耐药的An中受到损害。冈比亚株。建议监测吡丙醚化学品的抗药性。
Pyrethroid resistance is widespread in the malaria vector Anopheles gambiae leading to concerns about the future efficacy of bednets with pyrethroids as the sole active ingredient. The incorporation of pyriproxyfen (PPF), a juvenile hormone analogue, into pyrethroid treated bednets is being trialed in Africa. Pyrethroid resistance is commonly associated with elevated levels of P450 expression including CYPs 6M2, 6P2, 6P3, 6P4, 6P5, 6Z2 and 9J5. Having expressed these P450s in E. coli we find all are capable of metabolizing PPF. Inhibition of these P450s by permethrin, deltamethrin and PPF was also examined. Deltamethrin and permethrin were moderate inhibitors (IC50 1–10 μM) of diethoxyfluorescein (DEF) activity for all P450s apart from CYP6Z2 (IC50 > 10 μM), while PPF displayed weaker inhibition of all P450s (IC50 > 10 μM) except CYP's 6Z2 and 6P2 (IC50 1–10 μM). We found evidence of low levels of cross resistance between PPF and other insecticide classes by comparing the efficacy of PPF in inhibiting metamorphosis and inducing female sterility in an insecticide susceptible strain of An. gambiae and a multiple resistant strain from Cote d’Ivoire. Pyriproxyfen is metabolized by P450s associated with pyrethroid resistance. Pyrethroids may synergise pyriproxyfen activity. Pyriproxyfen efficacy is compromised in a multiple resistant An. gambiae strain. Resistance monitoring for pyriproxyfen chemistry is recommended.
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影响因子：
3.8
作者：
Abad-Franch F;Zamora-Perea E;Ferraz G;Padilla-Torres SD;Luz SL
通讯作者：
Luz SL
DOI：
10.1073/pnas.0901369106
发表时间：
2009-07-14
影响因子：
11.1
作者：
Devine, Gregor J.;Zamora Perea, Elvira;Morrison, Amy C.
通讯作者：
Morrison, Amy C.
影响因子：
11.8
作者：
Edi CV;Koudou BG;Jones CM;Weetman D;Ranson H
通讯作者：
Ranson H
影响因子：
3
作者：
Mbare, Oscar;Lindsay, Steven W.;Fillinger, Ulrike
通讯作者：
Fillinger, Ulrike
影响因子：
--
作者：
Krippendorff, Ben-Fillippo;Lienau, Philip;Huisinga, Wilhelm
通讯作者：
Huisinga, Wilhelm