Measurement of sterigmatocystin concentrations in urine for monitoring the contamination of cattle feed.
Measurement of sterigmatocystin concentrations in urine for monitoring the contamination of cattle feed.
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DOI：
10.3390/toxins6113117
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发表时间：
2014-11-04
期刊：
影响因子：
4.2
通讯作者：
Fink-Gremmels J
中科院分区：
文献类型：
--
作者：
Fushimi Y;Takagi M;Uno S;Kokushi E;Nakamura M;Hasunuma H;Shinya U;Deguchi E;Fink-Gremmels J
本研究旨在（1）测定牛饲料和尿液中真菌毒素杂色曲霉素（STC）的水平，（2）评价在饲料中添加真菌毒素吸附剂（MA）对尿液中STC浓度的影响。本研究中使用了两群雌性日本黑牛。每个牛群中的牛饲喂含有不同来源的稻草和标准浓缩物的标准日粮;每个牛群中的两组牛（每组n = 6）接受商业MA，与浓缩物混合或作为追肥，而第三组未接受补充剂并作为对照。在整个实验的不同时间点收集尿液和饲料样品。使用液相色谱-串联质谱法（LC-TMS）测量STC浓度。畜群1中秸秆中STC浓度（范围为0.15-0.24 mg/kg DM）高于畜群2（范围<0.01-0.06 mg/kg DM）。在牛群1中，更换污染饲料后2周，尿液中STC浓度显着下降，而MA补充没有影响。总之，尿液样本中的霉菌毒素是监测牛对多种霉菌毒素的全身暴露以及评估干预措施有效性的有用生物标志物。
This study aimed (1) at determining the levels of the fungal toxin sterigmatocystin (STC) in the feed and urine of cattle and (2) at evaluating the effects of supplementing the feed with a mycotoxin adsorbent (MA) on STC concentrations in urine. Two herds of female Japanese Black cattle were used in this study. The cattle in each herd were fed a standard ration containing rice straw from different sources and a standard concentrate; two groups of cattle from each herd (n = six per group) received the commercial MA, mixed with the concentrate or given as top-dressing, whereas a third group received no supplement and served as control. Urine and feed samples were collected at various time points throughout the experiment. STC concentrations were measured using liquid chromatography-tandem mass spectrometry (LC-TMS). STC concentrations in straw were higher in Herd 1 (range 0.15–0.24 mg/kg DM) than in Herd 2 (range <0.01–0.06 mg/kg DM). In Herd 1, STC concentrations in urine significantly declined 2 weeks after replacing the contaminated feed, whereas MA supplementation had no effect. In conclusion, mycotoxins in urine samples are useful biological markers for monitoring the systemic exposure of cattle to multiple mycotoxins, as well as evaluating the effectiveness of interventions.
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影响因子：
6.1
作者：
Warth, Benedikt;Parich, Alexandra;Krska, Rudolf
通讯作者：
Krska, Rudolf
影响因子：
2.8
作者：
Wilkinson, HH;Ramaswamy, A;Keller, NP
通讯作者：
Keller, NP
影响因子：
3.3
作者：
Hasunuma, H.;Takagi, M.;Fink-Gremmels, J.
通讯作者：
Fink-Gremmels, J.
影响因子：
3.3
作者：
Padilla, L.;Matsui, T.;Yano, H.
通讯作者：
Yano, H.
DOI：
10.1016/s0378-4347(00)82675-4
发表时间：
1989-09-29
期刊：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS
影响因子：
--
作者：
PRELUSKY, DB;WARNER, RM;TRENHOLM, HL
通讯作者：
TRENHOLM, HL