Air-blood barrier translocation of tracheally instilled gold nanoparticles inversely depends on particle size.
Air-blood barrier translocation of tracheally instilled gold nanoparticles inversely depends on particle size.
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DOI：
10.1021/nn403256v
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发表时间：
2014-01-28
期刊：
影响因子：
17.1
通讯作者：
Semmler-Behnke, Manuela
中科院分区：
文献类型：
--
作者：
Kreyling, Wolfgang G.;Hirn, Stephanie;Moeller, Winfried;Schleh, Carsten;Wenk, Alexander;Celik, Guelnaz;Lipka, Jens;Schaeffler, Martin;Haberl, Nadine;Johnston, Blair D.;Sperling, Ralph;Schmid, Guenter;Simon, Ulrich;Parak, Wolfgang J.;Semmler-Behnke, Manuela
关键词：
金纳米粒子（AuNP）在纳米医学的成像、诊断和治疗中提供了许多机会。为了评估金纳米粒子的生物学效应，我们将一套198金放射性标记的单分散的、表征良好的、带负电荷的五种不同尺寸（1.4、2.8、5、18、80、200 nm）的金纳米粒子和带正表面电荷的2.8 nm金纳米粒子注入大鼠体内。在1-h，3-h，和24-h的生物分布的金纳米粒子的定量测量γ-光谱法用于综合风险评估。我们的研究表明，随着AuNP变得越来越小，它们更有可能穿过空气-血液屏障（ABB），这在很大程度上取决于其金核的直径d−1的倒数;即它们的比表面积（SSA）。因此，1.4 nm AuNP（最高SSA）易位最多，而80 nm AuNP（最低SSA）易位最少，但200 nm颗粒不遵循d−1关系，易位显著高于80 nm AuNP。然而，相对于穿过ABB的AuNP，它们在大多数次级器官和组织中的保留不依赖于SSA。只有肾过滤，血液中的保留和通过尿液的排泄随着AuNP核心的d-1进一步下降。5、18和80 nm的AuNP在1 h后几乎完全易位，而1.4 nm的AuNP继续易位直到3 h。带负电荷的2.8 nm AuNP的易位显著高于带正电荷的2.8 nm AuNP。我们的研究表明，在ABB和积累和保留在次级器官和组织中的易位是两个不同的过程，都具体取决于粒子的特性，如SSA和表面电荷。
Gold nanoparticles (AuNP) provide many opportunities in imaging, diagnostics, and therapy in nanomedicine. For the assessment of AuNP biokinetics, we intratracheally instilled into rats a suite of 198Au-radio-labelled monodisperse, well-characterized, negatively-charged AuNP of five different sizes (1.4, 2.8, 5, 18, 80, 200 nm) and 2.8 nm AuNP with positive surface charges. At 1-h, 3-h, and 24-h the biodistribution of the AuNP was quantitatively measured by gamma-spectrometry to be used for comprehensive risk assessment. Our study shows, as AuNP get smaller, they are more likely to cross the air-blood-barrier (ABB) depending strongly on the inverse diameter d−1 of their gold core; i.e. their specific surface area (SSA). So, 1.4 nm AuNP (highest SSA) translocated most while 80 nm AuNP (lowest SSA) translocated least, but 200 nm particles did not follow the d−1 relation translocating significantly higher than 80 nm AuNP. However, relative to the AuNP which had crossed the ABB, their retention in most of the secondary organs and tissues was SSA-independent. Only renal filtration, retention in blood and excretion via urine further declined with d−1 of AuNP core. Translocation of 5, 18 and 80 nm AuNP is virtually complete after 1-h, while 1.4 nm AuNP continue to translocate until 3-h. Translocation of negatively charged 2.8 nm AuNP was significantly higher than for positively charged 2.8 nm AuNP. Our study shows that translocation across the ABB and accumulation and retention in secondary organs and tissues are two distinct processes, both depending specifically on particle characteristics such as SSA and surface charge.
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影响因子：
17.1
作者：
Lundqvist, Martin;Stigler, Johannes;Dawson, Kenneth
通讯作者：
Dawson, Kenneth
DOI：
10.1073/pnas.1220143110
发表时间：
2013-05-14
影响因子：
11.1
作者：
Leifert, Annika;Pan, Yu;Simon, Ulrich
通讯作者：
Simon, Ulrich
影响因子：
5.8
作者：
Brown, C. L.;Whitehouse, M. W.;Bushell, G. R.
通讯作者：
Bushell, G. R.
影响因子：
18.3
作者：
Kreyling, Wolfgang G.;Semmler-Behnke, Manuela;Takenaka, Shinji;Moeller, Winfried
通讯作者：
Moeller, Winfried
DOI：
10.1111/j.1748-1716.1993.tb09466.x
发表时间：
1993-01-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
--
作者：
JOHNSSON, E;HARALDSSON, B
通讯作者：
HARALDSSON, B