Comparison of long noncoding RNA and mRNA expression profiles in mesenchymal stem cells derived from human periodontal ligament and bone marrow.
Comparison of long noncoding RNA and mRNA expression profiles in mesenchymal stem cells derived from human periodontal ligament and bone marrow.
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人牙周膜和骨髓来源的间充质干细胞中长非编码 RNA 和 mRNA 表达谱的比较
DOI：
10.1155/2014/317853
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发表时间：
2014
影响因子：
--
通讯作者：
Fan Z
中科院分区：
文献类型：
--
作者：
Dong R;Du J;Wang L;Wang J;Ding G;Wang S;Fan Z
间充质干细胞（Mesenchymal stem cells，MSCs）在不同的解剖位置具有不同的生物学活性。维持多能状态和分化取决于数千个基因的表达和调控，但目前尚不清楚MSC多样性的分子机制。因此，潜在的MSC应用受到限制。长链非编码RNA（lncRNA）参与细胞过程的复杂分子回路。本研究采用lncRNA微阵列分析和生物信息学分析方法，研究了骨髓干细胞（BMSCs）和牙周膜干细胞（PDLSCs）lncRNA和mRNA表达谱的差异。在PDLSC中，与BMSC相比，许多lncRNA显著上调（n = 457）或下调（n = 513）。此外，1，578个mRNA差异表达。这些基因涉及可能与MSC特性相关的细胞通路，包括凋亡、MAPK、细胞周期和Wnt信号通路。信号网络分析表明，磷脂酶C β 4，细丝蛋白B β，钙/钙调蛋白依赖性蛋白激酶II γ，和离子型谷氨酸受体，AMPA 1，具有最高的介数中心的显着基因中的差异基因图谱网络。PDLSC和BMSC的编码-非编码基因共表达网络之间的比较确定趋化因子（C-X-C基序）配体12为MSC生物学中的核心调节因子。这些结果提供了对MSC生物学机制的深入了解。
Mesenchymal stem cells (MSCs) in different anatomic locations possess diverse biological activities. Maintaining the pluripotent state and differentiation depend on the expression and regulation of thousands of genes, but it remains unclear which molecular mechanisms underlie MSC diversity. Thus, potential MSC applications are restricted. Long noncoding RNAs (lncRNAs) are implicated in the complex molecular circuitry of cellular processes. We investigated differences in lncRNA and mRNA expression profiles between bone marrow stem cells (BMSCs) and periodontal ligament stem cells (PDLSCs) with lncRNA microarray assays and bioinformatics analysis. In PDLSCs, numerous lncRNAs were significantly upregulated (n = 457) or downregulated (n = 513) compared to BMSCs. Furthermore, 1,578 mRNAs were differentially expressed. These genes implicated cellular pathways that may be associated with MSC characteristics, including apoptosis, MAPK, cell cycle, and Wnt signaling pathway. Signal-net analysis indicated that phospholipase C beta 4, filamin B beta, calcium/calmodulin-dependent protein kinase II gamma, and the ionotropic glutamate receptor, AMPA 1, had the highest betweenness centrality among significant genes in the differential gene profile network. A comparison between the coding-noncoding gene coexpression networks of PDLSCs and BMSCs identified chemokine (C-X-C motif) ligand 12 as a core regulatory factor in MSC biology. These results provided insight into the mechanisms underlying MSC biology.
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影响因子：
4.3
作者：
Kim, Su-Hwan;Kim, Kyoung-Hwa;Lee, Yong-Moo
通讯作者：
Lee, Yong-Moo
影响因子：
4.2
作者：
Hayashi, Ousuke;Katsube, Yoshihiro;Ito, Hiromoto
通讯作者：
Ito, Hiromoto
影响因子：
12.4
作者：
d'Aquino, R.;Graziano, A.;Papaccio, G.
通讯作者：
Papaccio, G.
影响因子：
4.3
作者：
Kawaguchi, H;Hirachi, A;Kurihara, H
通讯作者：
Kurihara, H
影响因子：
--
作者：
Caplan, AI
通讯作者：
Caplan, AI