Construction of chromosome segment substitution lines in peanut (Arachis hypogaea L.) using a wild synthetic and QTL mapping for plant morphology.
Construction of chromosome segment substitution lines in peanut (Arachis hypogaea L.) using a wild synthetic and QTL mapping for plant morphology.
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DOI：
10.1371/journal.pone.0048642
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Rami JF
中科院分区：
文献类型：
--
作者：
Fonceka D;Tossim HA;Rivallan R;Vignes H;Lacut E;de Bellis F;Faye I;Ndoye O;Leal-Bertioli SC;Valls JF;Bertioli DJ;Glaszmann JC;Courtois B;Rami JF
染色体片段置换系(CSSL)是一类功能强大的QTL定位群体，已被用于阐明野生物种有趣性状的分子基础。栽培花生是一种遗传多样性有限的异源四倍体。获取花生野生近缘种的遗传多样性是花生育种的一个重要目标。在本研究中，我们利用标记辅助回交策略，从野生人工合成的异源四倍体(A.ipaënsis×A.duanensis)4x与栽培的Fleur11品种的杂交中产生了122个CSSL。122个CSSL提供了广泛的花生基因组覆盖，目标野生染色体片段平均长度为39.2 cM。为了证明这些品系的实用性，在80个CSSL的子集上对4个性状进行了评估。共有28个品系与Fleur11表现出显著差异。共检测到42个QTL，其中14个控制植株生长习性，15个控制主茎高度，12个控制植株伸展，1个控制花色。在42个QTL中，37个位于基因组区域，3个被认为是推测的QTL。这一QTL分析的一个重要发现是，花生生长习性是一个复杂的性状，受几个不同效应的QTL控制。本研究建立的CSSL群体对于解释性状变异的分子基础是有效的，并将为花生科学界未来的QTL定位研究提供有用的信息。
Chromosome segment substitution lines (CSSLs) are powerful QTL mapping populations that have been used to elucidate the molecular basis of interesting traits of wild species. Cultivated peanut is an allotetraploid with limited genetic diversity. Capturing the genetic diversity from peanut wild relatives is an important objective in many peanut breeding programs. In this study, we used a marker-assisted backcrossing strategy to produce a population of 122 CSSLs from the cross between the wild synthetic allotetraploid (A. ipaënsis×A. duranensis)4x and the cultivated Fleur11 variety. The 122 CSSLs offered a broad coverage of the peanut genome, with target wild chromosome segments averaging 39.2 cM in length. As a demonstration of the utility of these lines, four traits were evaluated in a subset of 80 CSSLs. A total of 28 lines showed significant differences from Fleur11. The line×trait significant associations were assigned to 42 QTLs: 14 for plant growth habit, 15 for height of the main stem, 12 for plant spread and one for flower color. Among the 42 QTLs, 37 were assigned to genomic regions and three QTL positions were considered putative. One important finding arising from this QTL analysis is that peanut growth habit is a complex trait that is governed by several QTLs with different effects. The CSSL population developed in this study has proved efficient for deciphering the molecular basis of trait variations and will be useful to the peanut scientific community for future QTL mapping studies.
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影响因子：
5.3
作者：
Foncéka D;Hodo-Abalo T;Rivallan R;Faye I;Sall MN;Ndoye O;Fávero AP;Bertioli DJ;Glaszmann JC;Courtois B;Rami JF
通讯作者：
Rami JF
影响因子：
5.4
作者：
Risterucci, AM;Grivet, L;Lanaud, C
通讯作者：
Lanaud, C
影响因子：
3
作者：
Seijo, Guillermo;Lavia, Graciela I.;Moscone, Eduardo A.
通讯作者：
Moscone, Eduardo A.
DOI：
10.3146/i0095-3679-28-2-12
发表时间：
2001-07-01
期刊：
Peanut Science
影响因子：
--
作者：
Simpson, C. E.
通讯作者：
Simpson, C. E.
影响因子：
2.3
作者：
Simpson, CE;Starr, JL
通讯作者：
Starr, JL