Genetic mapping of wild introgressions into cultivated peanut: a way toward enlarging the genetic basis of a recent allotetraploid.
Genetic mapping of wild introgressions into cultivated peanut: a way toward enlarging the genetic basis of a recent allotetraploid.
复制标题
DOI：
10.1186/1471-2229-9-103
复制
发表时间：
2009-08-03
影响因子：
5.3
通讯作者：
Rami JF
中科院分区：
文献类型：
--
作者：
Foncéka D;Hodo-Abalo T;Rivallan R;Faye I;Sall MN;Ndoye O;Fávero AP;Bertioli DJ;Glaszmann JC;Courtois B;Rami JF
花生（Arachis hypogaea L.）在世界各地被广泛用作粮食和经济作物。它被认为是一个异源四倍体（2n = 4x = 40），起源于两个野生二倍体之间的单一杂交事件。最可能的假设是A。duranensis为A基因组的野生供体，A. Ipaënsis作为B基因组的野生供体。栽培种质的分子多态性水平较低，目前已发表的遗传连锁图谱很少。由于野生种和栽培种之间的生殖障碍以及进行大量杂交所遇到的技术困难，野生种质在育种计划中的利用很少受到关注。我们在这里报告了一个基于SSR的遗传图谱的发展和全基因组片段渐渗到栽培品种的背景分析，通过利用人工合成的双二倍体之间的A. duranensis和A.伊潘西斯共将298个位点定位在21个连锁群中，总图距为1843.7cM，相邻标记间的平均距离为6.1cM。在双二倍体亲本和栽培品种之间观察到的多态性水平与A. duranensis和A. Ipaënsis分别是A和B基因组的最可能供体。A和B基因组之间的同线性分析揭示了同源异型LG的总体良好的共线性。与二倍体和四倍体图谱的比较揭示了导致A和B基因组物种分化的进化力量，并提出了B基因组物种的分类问题。结构修饰，如染色体片段倒位和一个主要的易位事件之前，四倍体的栽培物种被揭示。携带所需供体片段的BC1F1和随后的BC2F1品系的标记辅助选择提供了一组提供野生基因渗入的最佳分布的品系，所述供体片段具有对栽培品种的背景的最佳可能的返回。开发的遗传图谱允许A和B基因组的同线性分析、与二倍体和四倍体图谱的比较以及从野生合成到栽培品种背景的渐渗片段的分析。本研究所获得的材料将有助于花生回交育种和CSSL育种群体的发展。
Peanut (Arachis hypogaea L.) is widely used as a food and cash crop around the world. It is considered to be an allotetraploid (2n = 4x = 40) originated from a single hybridization event between two wild diploids. The most probable hypothesis gave A. duranensis as the wild donor of the A genome and A. ipaënsis as the wild donor of the B genome. A low level of molecular polymorphism is found in cultivated germplasm and up to date few genetic linkage maps have been published. The utilization of wild germplasm in breeding programs has received little attention due to the reproductive barriers between wild and cultivated species and to the technical difficulties encountered in making large number of crosses. We report here the development of a SSR based genetic map and the analysis of genome-wide segment introgressions into the background of a cultivated variety through the utilization of a synthetic amphidiploid between A. duranensis and A. ipaënsis. Two hundred ninety eight (298) loci were mapped in 21 linkage groups (LGs), spanning a total map distance of 1843.7 cM with an average distance of 6.1 cM between adjacent markers. The level of polymorphism observed between the parent of the amphidiploid and the cultivated variety is consistent with A. duranensis and A. ipaënsis being the most probable donor of the A and B genomes respectively. The synteny analysis between the A and B genomes revealed an overall good collinearity of the homeologous LGs. The comparison with the diploid and tetraploid maps shed new light on the evolutionary forces that contributed to the divergence of the A and B genome species and raised the question of the classification of the B genome species. Structural modifications such as chromosomal segment inversions and a major translocation event prior to the tetraploidisation of the cultivated species were revealed. Marker assisted selection of BC1F1 and then BC2F1 lines carrying the desirable donor segment with the best possible return to the background of the cultivated variety provided a set of lines offering an optimal distribution of the wild introgressions. The genetic map developed, allowed the synteny analysis of the A and B genomes, the comparison with diploid and tetraploid maps and the analysis of the introgression segments from the wild synthetic into the background of a cultivated variety. The material we have produced in this study should facilitate the development of advanced backcross and CSSL breeding populations for the improvement of cultivated peanut.
登录
查看更多内容
影响因子：
5.3
作者：
Gimenes MA;Hoshino AA;Barbosa AV;Palmieri DA;Lopes CR
通讯作者：
Lopes CR
影响因子：
5.4
作者：
HALWARD, T;STALKER, HT;KOCHERT, G
通讯作者：
KOCHERT, G
影响因子：
5.4
作者：
Fulton, TM;BeckBunn, T;Tanksley, SD
通讯作者：
Tanksley, SD
影响因子：
1.9
作者：
He, GH;Prakash, CS
通讯作者：
Prakash, CS
影响因子：
5.3
作者：
Cuc, Luu M.;Mace, Emma S.;Crouch, Jonathan H.;Quang, Vu D.;Long, Tran D.;Varshney, Rajeev K.
通讯作者：
Varshney, Rajeev K.