Culex quinquefasciatus from Rio de Janeiro Is Not Competent to Transmit the Local Zika Virus.
Culex quinquefasciatus from Rio de Janeiro Is Not Competent to Transmit the Local Zika Virus.
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DOI：
10.1371/journal.pntd.0004993
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发表时间：
2016-09
影响因子：
3.8
通讯作者：
Lourenço-de-Oliveira R
中科院分区：
文献类型：
--
作者：
Fernandes RS;Campos SS;Ferreira-de-Brito A;Miranda RM;Barbosa da Silva KA;Castro MG;Raphael LM;Brasil P;Failloux AB;Bonaldo MC;Lourenço-de-Oliveira R
自2015年5月巴西首次发现寨卡病毒（ZIKV）以来，美洲地区遭受了寨卡病毒的严重流行。属于伊蚊 Stegomyia 亚属的蚊子，特别是埃及伊蚊，被认为是 ZIKV 的主要传播媒介。然而，病毒在整个非洲大陆的迅速传播引起了人们对传播动态的一些担忧，特别是对潜在的蚊子媒介的担忧。这项工作的目的是评估来自巴西里约热内卢寨卡流行区的致倦库蚊 (Culex quinquefasciatus) 对当地流行的寨卡病毒分离株的媒介能力。致倦库蚊和伊蚊。来自里约热内卢的埃及伊蚊（ZIKV 感染阳性对照）经口暴露于从里约热内卢人类病例中分离出的两种 ZIKV 毒株（Rio-U1 和 Rio-S1）。将完全充血的蚊子放在培养箱中，温度为 26±1°C，12 小时：12 小时光：暗循环，湿度为 70±10%。对于每个组合蚊子种群（ZIKV 毒株），在病毒暴露后 7、14 和 21 天，通过分别分析身体（胸部加腹部）、头部和唾液，检查 30 个样本的感染、传播和传播率。所有 Cx 中的感染率都很低甚至完全不存在。 quinquefasciatus-病毒组合，并且白伊伊蚊的含量显着高。埃及伊蚊。此外，在任何 Cx 中均未检测到传播和传播。无论潜伏期和 ZIKV 分离株如何，致倦昆奎蚊都会被感染。相比之下，艾.埃及伊蚊确保了病毒的高度传播和中度至极高的传播。南方家蚊Cx。来自里约热内卢的 quinquefasciatus 不具备传播 ZIKV 本地毒株的能力。因此，没有实验证据表明Cx。 quinquefasciatus 可能在 ZIKV 传播中发挥了作用。因此，至少在里约，减少寨卡病毒传播的蚊子控制仍应重点关注白伊蚊。埃及伊蚊。寨卡疫情几乎影响到所有美洲国家。病原体是一种源自非洲的蚊媒病毒，传播到亚洲，最近又传播到太平洋地区和美洲。我们通过实验证明，来自里约热内卢的常见夜间叮咬蚊子库蚊（Culex quinquefasciatus）对当地传播的寨卡病毒（ZIKV）菌株不敏感。在 Cx 中未观察到传播。无论使用何种 ZIKV 分离株以及摄入受感染血粉后的潜伏期如何，致死致死昆克杆菌都会被感染。在 4 名 Cx 的唾液中未检测到传染性 ZIKV 颗粒。直到病毒暴露后 3 周才对 quinquefasciatus 群体进行检查。相比之下，我们证实当地埃及伊蚊可以被感染，以极高的比率传播 ZIKV，并在病毒暴露第 14 天后确保中度至极高的病毒传播。我们得出的结论是 Cx。 quinquefasciatus 不具备传播本地 ZIKV 的能力。我们的结果支持蚊子控制应重点关注白伊蚊。埃及伊蚊以减少寨卡病毒传播。
The Americas have suffered a dramatic epidemic of Zika since May in 2015, when Zika virus (ZIKV) was first detected in Brazil. Mosquitoes belonging to subgenus Stegomyia of Aedes, particularly Aedes aegypti, are considered the primary vectors of ZIKV. However, the rapid spread of the virus across the continent raised several concerns about the transmission dynamics, especially about potential mosquito vectors. The purpose of this work was to assess the vector competence of the house mosquito Culex quinquefasciatus from an epidemic Zika area, Rio de Janeiro, Brazil, for local circulating ZIKV isolates. Culex quinquefasciatus and Ae. aegypti (positive control of ZIKV infection) from Rio de Janeiro were orally exposed to two ZIKV strains isolated from human cases from Rio de Janeiro (Rio-U1 and Rio-S1). Fully engorged mosquitoes were held in incubators at 26 ± 1°C, 12 h:12 h light:dark cycle and 70 ± 10% humidity. For each combination mosquito population—ZIKV strain, 30 specimens were examined for infection, dissemination and transmission rates, at 7, 14 and 21 days after virus exposure by analyzing body (thorax plus abdomen), head and saliva respectively. Infection rates were minimal to completely absent in all Cx. quinquefasciatus-virus combinations and were significantly high for Ae. aegypti. Moreover, dissemination and transmission were not detected in any Cx. quinquefasciatus mosquitoes whatever the incubation period and the ZIKV isolate. In contrast, Ae. aegypti ensured high viral dissemination and moderate to very high transmission. The southern house mosquito Cx. quinquefasciatus from Rio de Janeiro was not competent to transmit local strains of ZIKV. Thus, there is no experimental evidence that Cx. quinquefasciatus likely plays a role in the ZIKV transmission. Consequently, at least in Rio, mosquito control to reduce ZIKV transmission should remain focused on Ae. aegypti. The pandemic Zika epidemic has affected nearly all American countries. The etiological agent is a mosquito-borne-virus originated from Africa that spread to Asia and more recently, to the Pacific region and the Americas. We experimentally demonstrated that the common house nightly biting mosquito Culex quinquefasciatus from Rio de Janeiro was not susceptible to locally circulating Zika virus (ZIKV) strains. Dissemination was not observed in Cx. quinquefasciatus regardless of the ZIKV isolate used and the incubation period after the ingestion of an infected blood meal. No infectious ZIKV particle was detected in the saliva of the four Cx. quinquefasciatus populations examined until 3 weeks after virus exposure. In contrast, we confirmed that local Aedes aegypti mosquitoes can be infected, disseminate ZIKV at significantly high rates, and assured moderate to very high viral transmission after day 14 of virus exposure. We concluded that Cx. quinquefasciatus is not competent to transmit local ZIKV. Our results support that mosquito control should focus on Ae. aegypti to reduce Zika transmission.
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DOI：
10.1126/science.aaf5036
发表时间：
2016-04-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Faria NR;Azevedo RDSDS;Kraemer MUG;Souza R;Cunha MS;Hill SC;Thézé J;Bonsall MB;Bowden TA;Rissanen I;Rocco IM;Nogueira JS;Maeda AY;Vasami FGDS;Macedo FLL;Suzuki A;Rodrigues SG;Cruz ACR;Nunes BT;Medeiros DBA;Rodrigues DSG;Queiroz ALN;da Silva EVP;Henriques DF;da Rosa EST;de Oliveira CS;Martins LC;Vasconcelos HB;Casseb LMN;Simith DB;Messina JP;Abade L;Lourenço J;Alcantara LCJ;de Lima MM;Giovanetti M;Hay SI;de Oliveira RS;Lemos PDS;de Oliveira LF;de Lima CPS;da Silva SP;de Vasconcelos JM;Franco L;Cardoso JF;Vianez-Júnior JLDSG;Mir D;Bello G;Delatorre E;Khan K;Creatore M;Coelho GE;de Oliveira WK;Tesh R;Pybus OG;Nunes MRT;Vasconcelos PFC
通讯作者：
Vasconcelos PFC
影响因子：
3.7
作者：
Diagne CT;Diallo D;Faye O;Ba Y;Faye O;Gaye A;Dia I;Faye O;Weaver SC;Sall AA;Diallo M
通讯作者：
Diallo M
影响因子：
3.8
作者：
Faye O;Freire CC;Iamarino A;Faye O;de Oliveira JV;Diallo M;Zanotto PM;Sall AA
通讯作者：
Sall AA
影响因子：
3.8
作者：
Bonaldo MC;Ribeiro IP;Lima NS;Dos Santos AA;Menezes LS;da Cruz SO;de Mello IS;Furtado ND;de Moura EE;Damasceno L;da Silva KA;de Castro MG;Gerber AL;de Almeida LG;Lourenço-de-Oliveira R;Vasconcelos AT;Brasil P
通讯作者：
Brasil P
影响因子：
4.5
作者：
Fansiri T;Fontaine A;Diancourt L;Caro V;Thaisomboonsuk B;Richardson JH;Jarman RG;Ponlawat A;Lambrechts L
通讯作者：
Lambrechts L