Injectable, high-density collagen gels for annulus fibrosus repair: An in vitro rat tail model.
Injectable, high-density collagen gels for annulus fibrosus repair: An in vitro rat tail model.
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DOI：
10.1002/jbm.a.35388
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发表时间：
2015-08
影响因子：
4.9
通讯作者：
Bonassar, Lawrence J.
中科院分区：
文献类型：
--
作者：
Borde, Brandon;Grunert, Peter;Haertl, Roger;Bonassar, Lawrence J.
当椎间盘组织通过受损的纤维环移位时，突出的椎间盘通常会引起背部疼痛。目前，纤维环修复的唯一方法是机械闭合缺损区，这对解决受损组织的生物愈合作用很小。胶原水凝胶是可注射的，已被用于修复体内的环状缺损。本研究用5、10和15 mg/ml的高密度胶原水凝胶修复体外培养的大鼠尾侧髓核损伤。一组15 mg/ml的凝胶与0.03 mM、0.07 mM或0.10 mM的核黄素进行交联。这些交联型、高密度的胶原凝胶在负载下保持了缺损处的存在，并对受损椎间盘的机械响应做出了积极的贡献。圆盘表现出95%未受损的有效平衡和瞬时模数的增加，以及受损圆盘的有效水力渗透率最多降低四倍。这些数据表明，高密度胶原蛋白凝胶可能有效地恢复受损椎间盘的机械功能，以及潜在的输送生物制剂的载体，如细胞或生长因子，可能有助于纤维环的修复。
A herniated intervertebral disc often causes back pain when disc tissue is displaced through a damaged annulus fibrosus. Currently the only methods available for annulus fibrosus repair involve mechanical closure of defect, which does little to address biological healing in the damaged tissue. Collagen hydrogels are injectable and have been used to repair annulus defects in vivo. In this study, high-density collagen hydrogels at 5, 10 and 15 mg/ml were used to repair defects made to intact rat caudal intervertebral discs in vitro. A group of gels at 15 mg/ml were also crosslinked with riboflavin at 0.03 mM, 0.07 mM or 0.10 mM . These crosslinked, high-density collagen gels maintained presence in the defect under loading and contributed positively to the mechanical response of damaged discs. Discs exhibited increases to 95% of undamaged effective equilibrium and instantaneous moduli as well as up to four fold decreases in effective hydraulic permeability from the damaged discs. These data suggest that high density collagen gels may be effective at restoring mechanical function of injured discs as well as potential vehicles for delivery of biological agents such as cells or growth factors that may aid in the repair of the annulus fibrosus.
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