Functional complexity of the axonal growth cone: a proteomic analysis.
Functional complexity of the axonal growth cone: a proteomic analysis.
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DOI：
10.1371/journal.pone.0031858
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Pfenninger KH
中科院分区：
文献类型：
--
作者：
Estrada-Bernal A;Sanford SD;Sosa LJ;Simon GC;Hansen KC;Pfenninger KH
生长锥，即新生神经突的尖端，在建立发育中的神经系统的布线中起着至关重要的作用。我们进行了广泛的蛋白质组学分析的轴突生长锥从胎儿Sprague-Dawley大鼠的大脑。在≥99%置信水平下鉴定了约2000种蛋白质。利用信息学，包括功能注释簇和KEGG通路分析，我们发现了参与轴突寻路，细胞骨架重塑，囊泡交通和碳水化合物代谢的蛋白质的巨大多样性，正如预期的那样。我们还发现了大量复杂的蛋白质参与翻译，蛋白质折叠，翻译后加工和蛋白酶体/泛素依赖性降解。对培养的海马神经元进行的免疫荧光研究证实了这些过程的蛋白质代表轴突生长锥中的存在。这些分析也提供了证据，粗面内质网，并揭示了一个网状结构配备高尔基体样功能的轴突生长锥。此外，Western blot显示，相对于胎脑匀浆，生长锥富集了一些参与蛋白质合成、折叠和catenate的蛋白质。我们的研究为进一步的研究提供了资源，并放大了相对最近发展的概念，即轴突生长锥配备了能够执行高度多样化功能的蛋白质。
The growth cone, the tip of the emerging neurite, plays a crucial role in establishing the wiring of the developing nervous system. We performed an extensive proteomic analysis of axonal growth cones isolated from the brains of fetal Sprague-Dawley rats. Approximately 2000 proteins were identified at ≥99% confidence level. Using informatics, including functional annotation cluster and KEGG pathway analysis, we found great diversity of proteins involved in axonal pathfinding, cytoskeletal remodeling, vesicular traffic and carbohydrate metabolism, as expected. We also found a large and complex array of proteins involved in translation, protein folding, posttranslational processing, and proteasome/ubiquitination-dependent degradation. Immunofluorescence studies performed on hippocampal neurons in culture confirmed the presence in the axonal growth cone of proteins representative of these processes. These analyses also provide evidence for rough endoplasmic reticulum and reveal a reticular structure equipped with Golgi-like functions in the axonal growth cone. Furthermore, Western blot revealed the growth cone enrichment, relative to fetal brain homogenate, of some of the proteins involved in protein synthesis, folding and catabolism. Our study provides a resource for further research and amplifies the relatively recently developed concept that the axonal growth cone is equipped with proteins capable of performing a highly diverse range of functions.
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影响因子：
16.2
作者：
Drinjakovic J;Jung H;Campbell DS;Strochlic L;Dwivedy A;Holt CE
通讯作者：
Holt CE
影响因子：
4.5
作者：
Gumy, Laura F.;Yeo, Giles S. H.;Fawcett, James W.
通讯作者：
Fawcett, James W.
DOI：
10.1016/0165-3806(87)90191-x
发表时间：
1987-07-01
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
FERREIRA, A;BUSCIGLIO, J;CACERES, A
通讯作者：
CACERES, A
DOI：
10.1073/pnas.0607394104
发表时间：
2006-12-26
影响因子：
11.1
作者：
Bi, Jing;Tsai, Nien-Pei;Wei, Li-Na
通讯作者：
Wei, Li-Na
影响因子：
16.2
作者：
Campbell, DS;Holt, CE
通讯作者：
Holt, CE