L-Glutamate supplementation improves small intestinal architecture and enhances the expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets.
L-Glutamate supplementation improves small intestinal architecture and enhances the expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets.
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DOI：
10.1371/journal.pone.0111950
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhou G
中科院分区：
文献类型：
--
作者：
Lin M;Zhang B;Yu C;Li J;Zhang L;Sun H;Gao F;Zhou G
谷氨酸是小肠的主要氧化燃料。然而，很少有研究表明l -谷氨酸对小肠结构和小肠氨基酸信号传导的影响。本试验旨在研究饲粮中添加l -谷氨酸对断奶仔猪肠道结构及空肠黏膜氨基酸受体和转运蛋白表达的影响。试验选用120头35±1日龄、平均体重为8.91±0.45 kg的断奶仔猪，随机分为2个处理，每6个重复，每个重复10头仔猪，分别饲喂含1.21%丙氨酸和2%谷氨酸的饲粮。补充l -谷氨酸可提高空肠黏膜谷氨酸草酰乙酸转氨酶（GOT）活性。添加l -谷氨酸可提高空肠黏膜谷氨酰胺合成酶（GS） mRNA表达量。饲粮中添加l -谷氨酸增加了十二指肠和空肠段绒毛高度，提高了空肠黏膜occludin和occludens蛋白-1 (ZO-1) mRNA相对表达量。补充l -谷氨酸增加了谷氨酸、精氨酸、组氨酸、异亮氨酸、亮氨酸、蛋氨酸、苯丙氨酸和苏氨酸的血浆浓度。补充l -谷氨酸还增加了空肠黏膜Ca2+敏感受体（CaR）、代谢性谷氨酸受体1 （mGluR1）和代谢性谷氨酸受体4 （mGluR4）以及中性氨基酸转运蛋白b0样蛋白（SLC1A5） mRNA的相对表达量。综上所述，饲粮中添加2% l -谷氨酸可改善断奶仔猪肠道完整性，影响空肠氨基酸受体和转运蛋白的表达，有利于改善空肠营养物质的消化吸收。
L-Glutamate is a major oxidative fuel for the small intestine. However, few studies have demonstrated the effect of L-glutamate on the intestinal architecture and signaling of amino acids in the small intestine. The aim of this study was to investigate the effects of dietary L-glutamate supplementation on the intestinal architecture and expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets. A total of 120 weaning piglets aged 35±1 days with an average body weight at 8.91±0.45 kg were randomly allocated to two treatments with six replicates of ten piglets each, fed with diets containing 1.21% alanine, or 2% L-glutamate. L-Glutamate supplementation increased the activity of glutamate oxaloacetate transaminase (GOT) in the jejunal mucosa. Also, the mRNA expression level of jejunal mucosa glutamine synthetase (GS) was increased by L-glutamate supplementation. The height of villi in duodenal and jejunal segments, and the relative mRNA expression of occludin and zonula occludens protein-1 (ZO-1) in jejunal mucosa were increased by dietary L-glutamate supplementation. L-Glutamate supplementation increased plasma concentrations of glutamate, arginine, histidine, isoleucine, leucine, methionine, phenylalanine and threonine. L-Glutamate supplementation also increased the relative mRNA expression of the jejunal mucosa Ca2+-sensing receptor (CaR), metabotropic glutamate receptor 1 (mGluR1) and metabotropic glutamate receptor 4 (mGluR4), and neutral amino acid transporter B0-like (SLC1A5) in the jejunal mucosa. These findings suggest that dietary addition of 2% L-glutamate improves the intestinal integrity and influences the expression of amino acid receptors and transporters in the jejunum of weaning, which is beneficial for the improvement of jejunal nutrients for digestion and absorption.
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影响因子：
3.3
作者：
Mace, O. J.;Marshall, F.
通讯作者：
Marshall, F.
影响因子：
7.1
作者：
Burrin, Douglas G.;Stoll, Barbara
通讯作者：
Stoll, Barbara
L-谷氨酰胺或 L-丙氨酰-L-谷氨酰胺可防止氧化剂或内毒素诱导的新生儿肠上皮细胞死亡
影响因子：
3.5
作者：
Haynes, Tony E.;Li, Peng;Wu, Guoyao
通讯作者：
Wu, Guoyao
DOI：
10.1152/ajpgi.00299.2010
发表时间：
2011-01-01
影响因子：
4.5
作者：
Boutry, Claire;Matsumoto, Hideki;Bos, Cecile
通讯作者：
Bos, Cecile
影响因子：
3.5
作者：
Li, Xilong;Rezaei, Reza;Wu, Guoyao
通讯作者：
Wu, Guoyao