Protective effects of zymosan on heat stress-induced immunosuppression and apoptosis in dairy cows and peripheral blood mononuclear cells
Protective effects of zymosan on heat stress-induced immunosuppression and apoptosis in dairy cows and peripheral blood mononuclear cells
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酵母聚糖对热应激引起的奶牛免疫抑制和细胞凋亡及外周血单个核细胞的保护作用
DOI：
10.1007/s12192-018-0916-z
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发表时间：
2018-06
影响因子：
3.8
通讯作者：
Huang Kehe
中科院分区：
文献类型：
--
作者：
Sun Yuhang;Liu Jin;Ye Gengping;Gan Fang;Hamid Mohammed;Liao Shengfa;Huang Kehe
热应激（HS）奶牛的生产性能和免疫力下降，但热休克蛋白表达和细胞凋亡增加。酵母多糖是一种从酵母细胞壁中提取的物质，已被证明可以调节免疫反应和防御氧化应激。然而，酵母多糖对HS条件下奶牛免疫功能及其他指标的影响的研究文献很少。本研究通过建立奶牛外周血单个核细胞（PBMC）模型和奶牛模型，研究酵母多糖对HS条件下奶牛生产性能、免疫功能、热休克蛋白及相关基因表达的影响。体外研究表明，热暴露可使奶牛PBMC增殖、IL-2产生和Bcl-2/Bax-α比值降低，而hsp 70 mRNA和蛋白表达及Annexin V-bing增加。而酵母多糖则显著逆转了HS诱导的上述变化。在体内试验中，随机选取40头荷斯坦奶牛，分为酵母多糖组（添加酵母多糖;n= 20）和对照组（不添加酵母多糖;n= 20）。结果表明，酵母多糖显著提高了HS奶牛的干物质采食量和产奶量，提高了血清伊加、IL-2和肿瘤坏死因子-α（TNF-α）含量，降低了HS奶牛的呼吸频率和Hepatichsp 70表达，提高了HS奶牛肝脏Bcl-2/Bax-α比值。因此，酵母多糖可以缓解HS诱导的免疫抑制和细胞凋亡，显著提高HS奶牛的生产性能和免疫力。
Dairy cows exposed to heat stress (HS) show decreased performance and immunity, but increased heat shock protein expressions and apoptosis. Zymosan, an extract from yeast cell walls, has been shown to modulate immune responses and defense against oxidative stress. However, few literatures are available about the effects of zymosan on immune responses and other parameters of the dairy cows under HS. Here, both primary peripheral blood mononuclear cell (PBMC) and dairy cow models were established to assess the effects of zymosan on performance, immunity, heat shock protein, and apoptosis-related gene expressions of dairy cows under HS. In vitro study showed that proliferation, IL-2 production, andBcl-2/Bax-αratio of cow primary PBMC were reduced, whereashsp70mRNA and protein expressions, as well as Annexin V-bing, were increased when PBMCs were exposed to heat. In contrast, zymosan significantly reversed these above changes induced by the HS. In the in vivo study, 40 Holstein dairy cows were randomly selected and assigned into zymosan group (supplemental zymosan;n= 20) and control group (no supplemental zymosan;n= 20). The results showed that zymosan improved significantly the dry matter intake and milk yield, increased IgA, IL-2, and tumor necrosis factor-α (TNF-α) contents in sera, as well as hepaticBcl-2/Bax-αratio, but decreased respiration rate and hepatichsp70expressions in the dairy cows under HS. Taken together, zymosan could alleviate HS-induced immunosuppression and apoptosis and improve significantly the productive performance and immunity of dairy cows under HS.
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DOI：
10.3390/molecules23030528
发表时间：
2018-02-27
期刊：
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