Transcriptional comparison of the filamentous fungus Neurospora crassa growing on three major monosaccharides D-glucose, D-xylose and L-arabinose.
Transcriptional comparison of the filamentous fungus Neurospora crassa growing on three major monosaccharides D-glucose, D-xylose and L-arabinose.
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在三种主要单糖 D-葡萄糖、D-木糖和 L-阿拉伯糖上生长的丝状真菌粗糙脉孢菌的转录比较
DOI：
10.1186/1754-6834-7-31
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发表时间：
2014-02-28
影响因子：
6.3
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Li J;Lin L;Li H;Tian C;Ma Y
背景：D-葡萄糖、D-木糖和L-阿拉伯糖是植物细胞壁中的三种主要单糖。所有三种糖的完全利用仍然是第二代纤维素分解生物乙醇生产的瓶颈，特别是L-阿拉伯糖。然而，鲜为人知的是基因表达谱在L-阿拉伯糖利用真菌和全基因组的真菌响应这三个主要单糖的比较尚未reported.Results：使用下一代测序技术，我们分析了转录组的N。在L-阿拉伯糖与D-木糖上生长的crassa，以D-葡萄糖作为参照。我们发现，L-阿拉伯糖上的基因表达谱显着不同的D-木糖。研究表明，L-阿拉伯糖可以广泛地改变真菌细胞的代谢途径，并诱导多种糖转运蛋白、半纤维素酶基因和转录因子的表达。相比之下，少得多的基因，主要与戊糖代谢途径，上调D-木糖。尽管碳饥饿反应是在L-阿拉伯糖上启动的，但与无碳条件下相比，对L-阿拉伯糖的重连代谢反应显著不同且更广泛。本文鉴定并表征了三种新型糖转运蛋白，包括一种葡萄糖转运蛋白GLT-1（NCU 01633）和两种新型戊糖转运蛋白XAT-1（NCU 01132）、XYT-1（NCU 05627）。一个转录因子与半纤维素酶基因的调控，HCR-1（NCU 05064）的特点也在本study.Conclusions：我们进行了第一次的转录组分析粗糙脉孢菌生长在L-阿拉伯糖和D-木糖和D-葡萄糖，这加深了了解的利用L-阿拉伯糖和D-木糖在丝状真菌上生长的细胞进行了比较分析。本研究所获得的数据集将有助于挖掘D-木糖和L-阿拉伯糖利用工程的靶基因，所鉴定的新型糖转运蛋白是戊糖利用和生物燃料生产的良好靶点。此外，半纤维素酶生产真菌可以提高通过修改半纤维素酶调节剂发现。
Background:D-glucose, D-xylose and L-arabinose are the three major monosaccharides in plant cell walls. Complete utilization of all three sugars is still a bottleneck for second-generation cellulolytic bioethanol production, especially for L-arabinose. However, little is known about gene expression profiles during L-arabinose utilization in fungi and a comparison of the genome-wide fungal response to these three major monosaccharides has not yet been reported.Results:Using next-generation sequencing technology, we have analyzed the transcriptome of N. crassa grown on L-arabinose versus D-xylose, with D-glucose as the reference. We found that the gene expression profiles on L-arabinose were dramatically different from those on D-xylose. It appears that L-arabinose can rewire the fungal cell metabolic pathway widely and provoke the expression of many kinds of sugar transporters, hemicellulase genes and transcription factors. In contrast, many fewer genes, mainly related to the pentose metabolic pathway, were upregulated on D-xylose. The rewired metabolic response to L-arabinose was significantly different and wider than that under no carbon conditions, although the carbon starvation response was initiated on L-arabinose. Three novel sugar transporters were identified and characterized for their substrates here, including one glucose transporter GLT-1 (NCU01633) and two novel pentose transporters, XAT-1 (NCU01132), XYT-1 (NCU05627). One transcription factor associated with the regulation of hemicellulase genes, HCR-1 (NCU05064) was also characterized in the present study.Conclusions:We conducted the first transcriptome analysis of Neurospora crassa grown on L-arabinose and performed a comparative analysis with cells grown on D-xylose and D-glucose, which deepens the understanding of the utilization of L-arabinose and D-xylose in filamentous fungi. The dataset generated by this research will be useful for mining target genes for D-xylose and L-arabinose utilization engineering and the novel sugar transportes identified are good targets for pentose untilization and biofuels production. Moreover, hemicellulase production by fungi could be improved by modifying the hemicellulase regulator discovered here.
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影响因子：
5
作者：
Battaglia, Evy;Hansen, Sara Fasmer;Leendertse, Anne;Madrid, Susan;Mulder, Harm;Nikolaev, Igor;de Vries, Ronald P.
通讯作者：
de Vries, Ronald P.
影响因子：
5.6
作者：
Bae, Brian;Sullivan, Ryan P.;Nair, Satish K.
通讯作者：
Nair, Satish K.
影响因子：
--
作者：
Du, Jing;Li, Sijin;Zhao, Huimin
通讯作者：
Zhao, Huimin
影响因子：
3.6
作者：
Benz JP;Chau BH;Zheng D;Bauer S;Glass NL;Somerville CR
通讯作者：
Somerville CR
影响因子：
3
作者：
Marui, J;Tanaka, A;Tsukagoshi, N
通讯作者：
Tsukagoshi, N