A method for facilitating the seed germination of a mycoheterotrophic orchid, Gastrodia pubilabiata, using decomposed leaf litter harboring a basidiomycete fungus, Mycena sp.
A method for facilitating the seed germination of a mycoheterotrophic orchid, Gastrodia pubilabiata, using decomposed leaf litter harboring a basidiomycete fungus, Mycena sp.
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DOI：
10.1186/s40529-017-0214-6
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发表时间：
2017-12-08
影响因子：
3.4
通讯作者：
Ogura-Tsujita Y
中科院分区：
文献类型：
--
作者：
Higaki K;Rammitsu K;Yamashita Y;Yukawa T;Ogura-Tsujita Y
真菌异养植物是最难保护的植物类群之一，因为它们完全依赖于共生真菌。建立可行的文化系统将大大有助于它们的保护。我们描述了一个简单的培养系统的真菌异养兰花，天麻pubilabiata，不需要实验室设施。兰花与分解落叶的真菌共生。 将毛唇天麻种子在装满从该物种的自然栖息地收集的落叶的塑料盒或玻璃瓶中孵育。35天后观察种子萌发，随后幼苗发育。从幼苗中分离到的真菌分离物被鉴定为小菇科（担子菌门），这是一种分解天麻属植物落叶的菌根真菌。我们的方法可以用来保护濒危的真菌异养植物与落叶分解真菌有效，也可以作为一个模式系统的生理和分子研究，这样的植物。本文的在线版本（10.1186/s40529-017-0214-6）包含补充材料，可供授权用户使用。
Mycoheterotrophic plants are one of the most difficult plant groups to conserve because they are entirely dependent on symbiotic fungi. Establishment of viable culture systems would greatly aid their conservation. We describe a simple culture system for the mycoheterotrophic orchid, Gastrodia pubilabiata, that does not require laboratory facilities. The orchid is symbiotic with leaf-litter-decomposing fungi. Gastrodia pubilabiata seeds were incubated in plastic boxes or glass bottles filled with leaf litter collected from the natural habitat of the species. Seed germination was observed after 35 days and seedling development followed. Fungal isolates from seedlings were identified as Mycenaceae (Basidiomycota), a leaf-litter-decomposing mycorrhizal fungus of Gastrodia species. Our method can be used to conserve endangered mycoheterotrophic plants associated with leaf litter-decomposing fungi efficiently, and can also serve as a model system for physiological and molecular studies of such plants. The online version of this article (10.1186/s40529-017-0214-6) contains supplementary material, which is available to authorized users.
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DOI：
10.1098/rspb.2008.1225
发表时间：
2009-02-22
影响因子：
4.7
作者：
Ogura-Tsujita, Yuki;Gebauer, Gerhard;Yukawa, Tomohisa
通讯作者：
Yukawa, Tomohisa
影响因子：
4.9
作者：
GARDES, M;BRUNS, TD
通讯作者：
BRUNS, TD
影响因子：
2.8
作者：
Yagame, Takahiro;Yamato, Masahide;Iwase, Koji
通讯作者：
Iwase, Koji
影响因子：
5.4
作者：
SUN, Y;SKINNER, DZ;HULBERT, SH
通讯作者：
HULBERT, SH
影响因子：
--
作者：
Brundrett, MC;Scade, A;Sivasithamparam, K
通讯作者：
Sivasithamparam, K