Modulation of gene expression in cold-induced sweetening resistant potato species Solanum berthaultii exposed to low temperature
Modulation of gene expression in cold-induced sweetening resistant potato species Solanum berthaultii exposed to low temperature
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低温诱导的抗甜马铃薯品种 Solanum berthaultii 基因表达的调节
DOI：
10.1007/s00438-012-0688-6
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发表时间：
2012-04
影响因子：
3.1
通讯作者：
Xie, Conghua
中科院分区：
文献类型：
--
作者：
He, Tianjiu;Lin, Yuan;Zhang, Huiling;Xie, Conghua
冷诱导甜化是影响马铃薯块茎加工品质的重要因素。为了更好地了解马铃薯顺式反应的分子事件以及不同品种马铃薯对顺式反应的不同敏感性，建立了抑制消减杂交库和基因芯片筛选系统。共发现188个差异表达基因(DE)在龙葵(Solanum Berthaultii)冷刺激下。这些功能基因主要与细胞拯救、防御和毒力、代谢、能量和蛋白质命运有关，包含在植物抵御非生物胁迫的各种过程中。利用qRT-PCR技术，以抗独联体(Ber)和独联体敏感的马铃薯(马铃薯3号，一种马铃薯品种)的冷藏块茎为材料，对这些DE基因的4种表达模式进行了分析。在这两种马铃薯块茎中，许多编码代谢相关蛋白的DE基因的表达模式和丰度不同，特别是与淀粉分解、蔗糖分解和糖酵解途径相关的基因，表明BER和E3对低温胁迫的响应具有不同的调控机制，这可能是马铃薯CIS的关键。对这些冷调控基因的深入研究将加深我们对马铃薯CIS调控机制的理解，为马铃薯加工品质的遗传改良提供直接途径。
Cold-induced sweetening (CIS) is a crucial factor influencing the processing quality of potato tubers. To better understand the molecular events of potato CIS and different CIS-sensitivity among various potato species, a suppression subtractive hybridization library and cDNA microarray gene filters were developed. A total of 188 genes were found to be differentially expressed (DE) in Solanum berthaultii (ber) upon cold stimulation. These functional genes were mostly related to cell rescue, defense and virulence, metabolism, energy and protein fate, included in various processes of plant defense against abiotic stresses. Four expression patterns of these DE genes were profiled by qRT-PCR using the cold-stored tubers of both CIS-resistant (ber) and CIS-sensitive (E-potato 3, a variety of S. tuberosum) potatoes. The expression pattern and abundance of many DE genes encoding proteins involved in metabolism were different in these two potato tubers, especially genes associated with amylolysis, sucrose decomposition and glycolysis pathways, indicating distinct regulatory mechanisms between ber and E3 in response to cold stress, which may be crucial for potato CIS. Further investigation of these cold-regulated genes will deepen our understanding of the regulatory mechanisms of potato CIS and direct approaches for the genetic improvement of potato processing quality.
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影响因子：
3.7
作者：
Boyko A;Blevins T;Yao Y;Golubov A;Bilichak A;Ilnytskyy Y;Hollunder J;Meins F Jr;Kovalchuk I
通讯作者：
Kovalchuk I
影响因子：
3.1
作者：
Liu, Xun;Zhang, Chi;Li, Xiu-Qing
通讯作者：
Li, Xiu-Qing
影响因子：
4.4
作者：
Bagnaresi P;Moschella A;Beretta O;Vitulli F;Ranalli P;Perata P
通讯作者：
Perata P
影响因子：
7.4
作者：
KIMMERER, TW;KOZLOWSKI, TT
通讯作者：
KOZLOWSKI, TT
DOI：
10.1073/pnas.0406674102
发表时间：
2005-02-01
影响因子：
11.1
作者：
Mikkelsen, R;Mutenda, KE;Blennow, A
通讯作者：
Blennow, A