Fecal Fingerprints of Enteric Pathogen Contamination in Public Environments of Kisumu, Kenya, Associated with Human Sanitation Conditions and Domestic Animals.
Fecal Fingerprints of Enteric Pathogen Contamination in Public Environments of Kisumu, Kenya, Associated with Human Sanitation Conditions and Domestic Animals.
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DOI：
10.1021/acs.est.8b01528
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发表时间：
2018-09-18
影响因子：
11.4
通讯作者：
Mumma J
中科院分区：
文献类型：
--
作者：
Baker KK;Senesac R;Sewell D;Sen Gupta A;Cumming O;Mumma J
幼儿在高疾病负担环境中感染各种肠道病原体，这表明环境中的病原体污染同样多种多样。本研究旨在表征肯尼亚基苏穆城市非正式住区公共领域肠道病原体污染的跨社区和社区内多样性，并评估病原体检测模式与人类和家畜卫生条件之间的关系。通过肠球菌检测和定量逆转录聚合酶链反应（qRT-PCR）检测了3个基苏穆社区166个公共场所土壤和地表水中19种肠道病原体的微生物污染。回归被用来评估观察到的肠球菌污染的卫生指标和病原体的存在和浓度，以及病原体的多样性之间的关联。在基苏穆公共场所共检出17种病原体。在33%的土壤和65%的地表水样品中共检测到肠道病原体。更大的病原体多样性与家畜粪便的存在有关，但与人类露天排便、厕所恶化、苍蝇或人类粪便的处理无关。卫生条件与肠球菌、特定病原体浓度或“任何病原体”无关。幼儿在40%的观察地点玩耍。可能需要管理家畜粪便，以减少高负担环境中的肠道病原体环境污染。
Young children are infected by a diverse range of enteric pathogens in high disease burden settings, suggesting pathogen contamination of the environment is equally diverse. This study aimed to characterize across- and within-neighborhood diversity in enteric pathogen contamination of public domains in urban informal settlements of Kisumu, Kenya, and to assess the relationship between pathogen detection patterns and human and domestic animal sanitation conditions. Microbial contamination of soil and surface water from 166 public sites in three Kisumu neighborhoods was measured by enterococcal assays and quantitative reverse transcription polymerase chain reaction (qRT-PCR) for 19 enteric pathogens. Regression was used to assess the association between observed sanitary indicators of contamination with enterococci and pathogen presence and concentration, and pathogen diversity. Seventeen types of pathogens were detected in Kisumu public domains. Enteric pathogens were codetected in 33% of soil and 65% of surface water samples. Greater pathogen diversity was associated with the presence of domestic animal feces but not with human open defecation, deteriorating latrines, flies, or disposal of human feces. Sanitary conditions were not associated with enterococcal bacteria, specific pathogen concentrations, or “any pathogen”. Young children played at 40% of observed sites. Managing domestic animal feces may be required to reduce enteric pathogen environmental contamination in high-burden settings.
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影响因子：
2.7
作者：
Haramoto, E.;Katayama, H.;Ohgaki, S.
通讯作者：
Ohgaki, S.
影响因子：
4.4
作者：
Desmarais, TR;Solo-Gabriele, HM;Palmer, CJ
通讯作者：
Palmer, CJ
影响因子：
12.8
作者：
Griffith, John F.;Cao, Yiping;Weisberg, Stephen B.
通讯作者：
Weisberg, Stephen B.
DOI：
10.1073/pnas.0705599104
发表时间：
2007-11-06
影响因子：
11.1
作者：
Alam, Munirul;Sultana, Marzia;Colwell, Rita R.
通讯作者：
Colwell, Rita R.
影响因子：
4.1
作者：
Ben Said, Leila;Klibi, Naouel;Torres, Carmen
通讯作者：
Torres, Carmen