Specific Oral Microbial Differences in Proteobacteria and Bacteroidetes Are Associated with Distinct Sites When Moving from Healthy Mucosa to Oral Dysplasia-A Microbiome and Gene Profiling Study and Focused Review.
Specific Oral Microbial Differences in Proteobacteria and Bacteroidetes Are Associated with Distinct Sites When Moving from Healthy Mucosa to Oral Dysplasia-A Microbiome and Gene Profiling Study and Focused Review.
复制标题
DOI：
10.3390/microorganisms11092250
复制
发表时间：
2023-09-07
期刊：
影响因子：
4.5
通讯作者：
Kapila YL
中科院分区：
文献类型：
--
作者：
Radaic A;Shamir ER;Jones K;Villa A;Garud NR;Tward AD;Kamarajan P;Kapila YL
口腔潜在恶性疾病（OPMD）是一组具有口腔鳞状细胞癌（OSCC）发展风险的疾病。最近的研究表明，牙周病相关的致病菌可能在从健康粘膜到异型增生和OSCC的转变中发挥作用。然而，与从健康粘膜到异型增生的转变相关的微生物特征尚未建立。为了表征这些不同部位的口腔微生物特征，我们对口腔拭子和福尔马林固定石蜡包埋组织（FFPE）样本进行了16 S测序分析。我们收集了健康粘膜（来自健康患者）、组织学上正常的异型增生附近粘膜和低度口腔异型增生的口腔拭子。此外，还收集了组织学正常的口腔鳞状细胞癌附近粘膜的FFPE样本，以及低级别和高级别的口腔异型增生样本。所收集的数据表明，在口腔粘膜，异型增生，和口腔鳞状细胞癌的不同部位的α和β微生物的dilibrium的显着差异，以及这些网站内的差异增加。我们发现，变形菌门的丰度增加，同时在厚壁菌门的丰度逐渐减少，以及改变水平的盲肠肠球菌，牙周梭杆菌，产黑色素普雷沃菌，和犬梭杆菌从健康到患病部位。此外，拭子样本分析表明，口腔微生物组可能在组织学正常的区域发生改变，包括在与发育异常相邻的粘膜中。此外，口腔拭子样本中特定微生物组变化的趋势先于组织中的变化，这意味着临床诊断的早期检测机会。此外，我们评估了牙龈卟啉单胞菌、T. Denticola，齿生F. nucelatum或S. sanguinis的研究发现，这三种牙周病原体丰富了与癌症进展相关的遗传过程，包括皮肤角质化/角化，而牙周组织丰富了与RNA加工和粘附相关的过程。最后，我们回顾了发育不良微生物组文献，发现链球菌属等肠道细菌显著减少，同时病原菌增加，主要是拟杆菌门和梭杆菌属。这些发现表明，口腔微生物组的特征可以作为异型增生和OSCC疾病进展的新生物标志物。
Oral potentially malignant disorders (OPMDs) are a group of conditions that carry a risk of oral squamous cell carcinoma (OSCC) development. Recent studies indicate that periodontal disease-associated pathogenic bacteria may play a role in the transition from healthy mucosa to dysplasia and to OSCC. Yet, the microbial signatures associated with the transition from healthy mucosa to dysplasia have not been established. To characterize oral microbial signatures at these different sites, we performed a 16S sequencing analysis of both oral swab and formalin-fixed, paraffin-embedded tissue (FFPE) samples. We collected oral swabs from healthy mucosa (from healthy patients), histologically normal mucosa adjacent to dysplasia, and low-grade oral dysplasia. Additionally, FFPE samples from histologically normal mucosa adjacent to OSCC, plus low grade and high-grade oral dysplasia samples were also collected. The collected data demonstrate significant differences in the alpha and beta microbial diversities of different sites in oral mucosa, dysplasia, and OSCC, as well as increased dissimilarities within these sites. We found that the Proteobacteria phyla abundance increased, concurrent with a progressive decrease in the Firmicutes phyla abundance, as well as altered levels of Enterococcus cecorum, Fusobacterium periodonticum, Prevotella melaninogenica, and Fusobacterium canifelinum when moving from healthy to diseased sites. Moreover, the swab sample analysis indicates that the oral microbiome may be altered in areas that are histologically normal, including in mucosa adjacent to dysplasia. Furthermore, trends in specific microbiome changes in oral swab samples preceded those in the tissues, signifying early detection opportunities for clinical diagnosis. In addition, we evaluated the gene expression profile of OSCC cells (HSC-3) infected with either P. gingivalis, T. denticola, F. nucelatum, or S. sanguinis and found that the three periodontopathogens enrich genetic processes related to cancer progression, including skin keratinization/cornification, while the commensal enriched processes related to RNA processing and adhesion. Finally, we reviewed the dysplasia microbiome literature and found a significant decrease in commensal bacteria, such as the Streptococci genus, and a simultaneous increase in pathogenic bacteria, mainly Bacteroidetes phyla and Fusobacterium genus. These findings suggest that features of the oral microbiome can serve as novel biomarkers for dysplasia and OSCC disease progression.
登录
查看更多内容
影响因子：
3.7
作者：
Abdalla Z;Walsh T;Thakker N;Ward CM
通讯作者：
Ward CM
DOI：
10.4103/jomfp.jomfp_89_16
发表时间：
2018-05
期刊：
Journal of oral and maxillofacial pathology : JOMFP
影响因子：
--
作者：
Gaonkar PP;Patankar SR;Tripathi N;Sridharan G
通讯作者：
Sridharan G
DOI：
10.1007/978-1-4939-3515-4_2
发表时间：
2016-01-01
期刊：
BACTERIAL THERAPY OF CANCER: METHODS AND PROTOCOLS
影响因子：
--
作者：
Fang, Jun;Long, Liao;Maeda, Hiroshi
通讯作者：
Maeda, Hiroshi
影响因子：
5.2
作者：
Amer A;Galvin S;Healy CM;Moran GP
通讯作者：
Moran GP
影响因子：
4.5
作者：
Gopinath D;Kunnath Menon R;Chun Wie C;Banerjee M;Panda S;Mandal D;Behera PK;Roychoudhury S;Kheur S;George Botelho M;Johnson NW
通讯作者：
Johnson NW