Salivary bacterial shifts in oral leukoplakia resemble the dysbiotic oral cancer bacteriome.
Salivary bacterial shifts in oral leukoplakia resemble the dysbiotic oral cancer bacteriome.
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口腔白斑中的唾液细菌移位类似于口腔癌病原菌群。
DOI：
10.1080/20002297.2020.1857998
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发表时间：
2020-12-09
影响因子：
4.5
通讯作者：
Johnson NW
中科院分区：
文献类型：
--
作者：
Gopinath D;Kunnath Menon R;Chun Wie C;Banerjee M;Panda S;Mandal D;Behera PK;Roychoudhury S;Kheur S;George Botelho M;Johnson NW
目的：虽然有些口腔癌似乎是从头产生的，但其他口腔癌是在口腔长期存在的情况下发展而来的，具有恶性潜力，现在称为口腔潜在恶性疾病 (OPMD)。与 OPMD 相关的口腔细菌组的研究程度低于与口腔癌相关的细菌组。为了详细描述这种关联，我们比较了口腔白斑、口腔癌和健康对照患者全口液 (WMF) 中的细菌组。方法：使用 Illumina MiSeq 平台对 20 名白斑患者、31 名口腔癌患者和 23 名健康对照者的 WMF 细菌组进行分析。使用 DADA2 处理测序读数，并使用系统发育放置方法进行分类学分类。使用稀疏偏最小二乘回归判别分析模型来识别最能区分研究群体的细菌分类群。结果：我们发现白斑和口腔癌的 WMF 细菌组之间存在相当大的重叠，同时观察到健康对照和前两种疾病之间有更清晰的分离。具体来说，分离归因于属于巨球菌属、未分类肠杆菌属、普雷沃菌属、卟啉单胞菌属、罗氏菌属和沙门氏菌属、链球菌属和梭杆菌属的 14 个分类群。白斑和口腔癌之间最具区别性的细菌属是巨球菌属、未分类肠杆菌属、沙门氏菌属和普雷沃氏菌属。结论：口腔细菌可能在口腔癌发生的早期阶段发挥作用，因为菌群失调与口腔白斑相关，并且与健康对照相比，这与口腔癌更相似。我们的研究结果可能对制定针对口腔癌发生的早期微生物驱动因素的口腔癌预防策略具有影响。
Objective: While some oral carcinomas appear to arise de novo, others develop within long-standing conditions of the oral cavity that have malignant potential, now known as oral potentially malignant disorders (OPMDs). The oral bacteriome associated with OPMD has been studied to a lesser extent than that associated with oral cancer. To characterize the association in detail we compared the bacteriome in whole mouth fluid (WMF) in patients with oral leukoplakia, oral cancer and healthy controls. Methods: WMF bacteriome from 20 leukoplakia patients, 31 patients with oral cancer and 23 healthy controls were profiled using the Illumina MiSeq platform. Sequencing reads were processed using DADA2, and taxonomical classification was performed using the phylogenetic placement method. Sparse Partial Least Squares Regression Discriminant Analysis model was used to identify bacterial taxa that best discriminate the studied groups. Results: We found considerable overlap between the WMF bacteriome of leukoplakia and oral cancer while a clearer separation between healthy controls and the former two disorders was observed. Specifically, the separation was attributed to 14 taxa belonging to the genera Megaspheara, unclassified enterobacteria, Prevotella, Porphyromonas, Rothia and Salmonella, Streptococcus, and Fusobacterium. The most discriminative bacterial genera between leukoplakia and oral cancer were Megasphaera, unclassified Enterobacteriae, Salmonella and Prevotella. Conclusion: Oral bacteria may play a role in the early stages of oral carcinogenesis as a dysbiotic bacteriome is associated with oral leukoplakia and this resembles that of oral cancer more than healthy controls. Our findings may have implications for developing oral cancer prevention strategies targeting early microbial drivers of oral carcinogenesis.
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影响因子：
--
作者：
Pushalkar S;Mane SP;Ji X;Li Y;Evans C;Crasta OR;Morse D;Meagher R;Singh A;Saxena D
通讯作者：
Saxena D
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
DOI：
10.1073/pnas.1820594116
发表时间：
2019-04-23
影响因子：
11.1
作者：
Baker, Jonathon L.;Hendrickson, Erik L.;Shi, Wenyuan
通讯作者：
Shi, Wenyuan
影响因子：
8.8
作者：
Gannot, G;Gannot, I;Keisari, Y
通讯作者：
Keisari, Y
影响因子：
3.7
作者：
Mughini-Gras, Lapo;Schaapveld, Michael;Neefjes, Jacques
通讯作者：
Neefjes, Jacques