Prp22 and spliceosome components regulate chromatin dynamics in germ-line polyploid cells.
Prp22 and spliceosome components regulate chromatin dynamics in germ-line polyploid cells.
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DOI：
10.1371/journal.pone.0079048
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Deng WM
中科院分区：
文献类型：
--
作者：
Klusza S;Novak A;Figueroa S;Palmer W;Deng WM
在果蝇卵子发生过程中，生殖系滋养细胞的内多倍体细胞核随着卵子发生的进展在形态上发生了巨大的变化;在卵子发生的早期阶段，容易看到的染色体最初是多线的，然后它们短暂地凝聚成一个独特的“5-blob”构型，随后分散成弥散状态。许多具有不同细胞功能的基因的突变可以影响滋养细胞完全解浓缩其染色质的能力，导致在卵子发生的整个后期阶段维持的“5-blob停滞”表型。然而，对乳细胞（NC）染色质分散的机制和意义仍然知之甚少。在这里，我们报告说，屏幕上的5-斑点表型的修改器在生殖系分离的剪接体基因花生，果蝇Prp 22。我们表明，减少剪接体活动，通过损失花生促进deconcensation缺陷NC核中期卵子发生。我们还表明，Prp 38剪接体蛋白积累在营养细胞的核质与花生功能受损，这表明剪接体回收受损。最后，我们发现，额外的spliceosomal蛋白的损失损害NC染色质在卵子发生的后期阶段的完全解凝聚，这表明，个别spliceosomal亚复合物调节表达的不同子集的基因所需的正确形态的内多倍体护士细胞。
During Drosophila oogenesis, the endopolyploid nuclei of germ-line nurse cells undergo a dramatic shift in morphology as oogenesis progresses; the easily-visible chromosomes are initially polytenic during the early stages of oogenesis before they transiently condense into a distinct ‘5-blob’ configuration, with subsequent dispersal into a diffuse state. Mutations in many genes, with diverse cellular functions, can affect the ability of nurse cells to fully decondense their chromatin, resulting in a ‘5-blob arrest’ phenotype that is maintained throughout the later stages of oogenesis. However, the mechanisms and significance of nurse-cell (NC) chromatin dispersal remain poorly understood. Here, we report that a screen for modifiers of the 5-blob phenotype in the germ line isolated the spliceosomal gene peanuts, the Drosophila Prp22. We demonstrate that reduction of spliceosomal activity through loss of peanuts promotes decondensation defects in NC nuclei during mid-oogenesis. We also show that the Prp38 spliceosomal protein accumulates in the nucleoplasm of nurse cells with impaired peanuts function, suggesting that spliceosomal recycling is impaired. Finally, we reveal that loss of additional spliceosomal proteins impairs the full decondensation of NC chromatin during later stages of oogenesis, suggesting that individual spliceosomal subcomplexes modulate expression of the distinct subset of genes that are required for correct morphology in endopolyploid nurse cells.
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