Condensin II promotes the formation of chromosome territories by inducing axial compaction of polyploid interphase chromosomes.
Condensin II promotes the formation of chromosome territories by inducing axial compaction of polyploid interphase chromosomes.
复制标题
DOI：
10.1371/journal.pgen.1002873
复制
发表时间：
2012
期刊：
影响因子：
4.5
通讯作者：
Bosco G
中科院分区：
文献类型：
--
作者：
Bauer CR;Hartl TA;Bosco G
真核生物的细胞核在空间和功能上都是分开的。这种组织有助于维持、表达和传递遗传信息。尽管近年来我们探测细胞核内基因组物理结构的能力有了很大的提高，但对调节其组织的因素或实现特定组织状态的机制知之甚少。在这里，我们发现黑腹果蝇凝缩蛋白II诱导间期染色体的轴向压实，全局破坏染色体间相互作用，并促进中心周围异染色质的分散。这些凝缩蛋白II的活动将细胞核划分为离散的染色体区域，并表明在有丝分裂和间期调节基因组空间结构的机制中存在共性。最近的一些研究已经推翻了染色体是作为细胞核内一团缠结的染色质纤维存在的观点。在包括哺乳动物在内的许多生物体中，每条染色体占据细胞核的特定区域，称为染色体区域。这种组织对许多生物学过程都有影响，比如在癌症中常见的染色体重排，以及控制基因表达方式的亚核结构之间的相互作用。尽管如此，人们对负责创造或维持染色体区域的基因或机制知之甚少。在这里，我们表明冷凝素II复合体可以诱导果蝇染色体区域的形成。我们认为这种活性源于凝缩蛋白II减少染色体长度的能力。
The eukaryotic nucleus is both spatially and functionally partitioned. This organization contributes to the maintenance, expression, and transmission of genetic information. Though our ability to probe the physical structure of the genome within the nucleus has improved substantially in recent years, relatively little is known about the factors that regulate its organization or the mechanisms through which specific organizational states are achieved. Here, we show that Drosophila melanogaster Condensin II induces axial compaction of interphase chromosomes, globally disrupts interchromosomal interactions, and promotes the dispersal of peri-centric heterochromatin. These Condensin II activities compartmentalize the nucleus into discrete chromosome territories and indicate commonalities in the mechanisms that regulate the spatial structure of the genome during mitosis and interphase. A number of recent studies have debunked the idea that chromosomes exist as a tangled mass of chromatin fibers within the nucleus. In many organisms, including mammals, each chromosome occupies a specific region of the nucleus known as a chromosome territory. This organization has implications for many biological processes such as chromosomal rearrangements that are common in cancer and the interactions between sub-nuclear structures that control how genes are expressed. Despite this, little is known about the genes or mechanisms that are responsible for creating or maintaining chromosome territories. Here, we show that the Condensin II complex can induce the formation of chromosome territories in fruit flies. We propose that this activity stems from the ability of Condensin II to reduce the length of chromosomes.
登录
查看更多内容
影响因子：
7.5
作者：
Cremer, Thomas;Cremer, Marion;Fakan, Stanislav
通讯作者：
Fakan, Stanislav
影响因子：
7.8
作者：
HOCHSTRASSER, M;SEDAT, JW
通讯作者：
SEDAT, JW
影响因子：
64.5
作者：
Kimura, K;Hirano, T
通讯作者：
Hirano, T
影响因子：
56.9
作者：
Hartl, Tom A.;Smith, Helen F.;Bosco, Giovanni
通讯作者：
Bosco, Giovanni
DOI：
10.1083/jcb.200908026
发表时间：
2010-02-22
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fazzio TG;Panning B
通讯作者：
Panning B