A Head-Mounted Camera System Integrates Detailed Behavioral Monitoring with Multichannel Electrophysiology in Freely Moving Mice.
A Head-Mounted Camera System Integrates Detailed Behavioral Monitoring with Multichannel Electrophysiology in Freely Moving Mice.
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DOI：
10.1016/j.neuron.2018.09.020
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发表时间：
2018-10-10
期刊：
影响因子：
16.2
通讯作者：
Linden JF
中科院分区：
文献类型：
--
作者：
Meyer AF;Poort J;O'Keefe J;Sahani M;Linden JF
在理解自然行为的神经基础方面的突破需要神经记录和干预与高保真的多模式行为监测相结合。广泛的神经回路解剖遗传工具包，以及发达的神经记录技术，使小鼠成为系统神经科学的强大模式生物。然而，大多数获取小鼠行为数据的高带宽方法依赖于固定位置的摄像头和其他非动物设备，这使得对自由从事自然行为的动物的监测变得复杂。在这里，我们报告了一种结合头部运动传感器的轻型头盔相机系统的开发，以同时监控自由行为的小鼠的眼睛位置、瞳孔扩大、搅拌和耳廓运动以及头部运动。通过观察将眼睛位置与头部方向联系起来；将快速刺激与非触觉刺激联系起来；在电生理实验中，将视觉皮质活动与头部随意运动联系起来，证明了这项组合技术的力量。在自由移动的小鼠系统中监测到的眼睛、胡须、头部和神经活动产生稳定的视频输出，并使小鼠的行为在很大程度上保持不变。在慢速和快时标的眼睛和头部运动之间的密切联系在黑暗中活跃的头部运动强烈地调节初级视觉皮质活动Meyer等人。描述一种新的头戴式摄像系统，它能够对自由活动的小鼠进行详细的行为监测和神经记录。该系统揭示了眼睛和头部运动之间的紧密耦合，以及初级视觉皮质中与头部运动相关的活动。
Breakthroughs in understanding the neural basis of natural behavior require neural recording and intervention to be paired with high-fidelity multimodal behavioral monitoring. An extensive genetic toolkit for neural circuit dissection, and well-developed neural recording technology, make the mouse a powerful model organism for systems neuroscience. However, most methods for high-bandwidth acquisition of behavioral data in mice rely upon fixed-position cameras and other off-animal devices, complicating the monitoring of animals freely engaged in natural behaviors. Here, we report the development of a lightweight head-mounted camera system combined with head-movement sensors to simultaneously monitor eye position, pupil dilation, whisking, and pinna movements along with head motion in unrestrained, freely behaving mice. The power of the combined technology is demonstrated by observations linking eye position to head orientation; whisking to non-tactile stimulation; and, in electrophysiological experiments, visual cortical activity to volitional head movements. Eyes, whiskers, head, and neural activity monitored in freely moving mice System generates stable video output and leaves mouse behavior largely unchanged Close link between eye and head movements at both slow and fast timescales Active head movements in the dark strongly modulate primary visual cortex activity Meyer et al. describe a new head-mounted camera system that enables detailed behavioral monitoring along with neural recording in freely moving mice. The system reveals close coupling between eye and head movements, and head-movement-related activity in primary visual cortex.
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