Effects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.
Effects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.
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DOI：
10.1371/journal.pone.0033791
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Klug G
中科院分区：
文献类型：
--
作者：
Frühwirth S;Teich K;Klug G
一些调节因子正在控制兼性光合细菌球形红杆菌中光合机构的形成。影响光合作用基因表达的蛋白质之一是蓝光光感受器隐花色素 CryB。这项研究探讨了 CryB 对整体基因表达的影响。数据显示，CryB 不仅影响光合作用基因的表达，还影响柠檬酸循环和氧化磷酸化等非光合作用能量代谢的基因。此外，一些参与RNA加工和转录调控的基因也受到cryB缺失的影响。尽管 CryB 被证明会经历光循环，但它不仅会影响蓝光照射下的基因表达，还会影响单线态氧应激条件下的基因表达。虽然这些反应有很大的重叠，但一些 CryB 依赖性效应是蓝光或光氧化应激所特有的。除了蛋白质编码基因外，一些 sRNA 基因也表现出 CryB 依赖性表达。这些发现为细菌隐花色素的功能提供了新的见解，并首次证明了其在氧化应激反应中的功能。
Several regulators are controlling the formation of the photosynthetic apparatus in the facultatively photosynthetic bacterium Rhodobacter sphaeroides. Among the proteins affecting photosynthesis gene expression is the blue light photoreceptor cryptochrome CryB. This study addresses the effect of CryB on global gene expression. The data reveal that CryB does not only influence photosynthesis gene expression but also genes for the non-photosynthetic energy metabolism like citric acid cycle and oxidative phosphorylation. In addition several genes involved in RNA processing and in transcriptional regulation are affected by a cryB deletion. Although CryB was shown to undergo a photocycle it does not only affect gene expression in response to blue light illumination but also in response to singlet oxygen stress conditions. While there is a large overlap in these responses, some CryB-dependent effects are specific for blue-light or photooxidative stress. In addition to protein-coding genes some genes for sRNAs show CryB-dependent expression. These findings give new insight into the function of bacterial cryptochromes and demonstrate for the first time a function in the oxidative stress response.
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