Overexpression of phyA and appA genes improves soil organic phosphorus utilisation and seed phytase activity in Brassica napus.
Overexpression of phyA and appA genes improves soil organic phosphorus utilisation and seed phytase activity in Brassica napus.
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DOI：
10.1371/journal.pone.0060801
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Xu F
中科院分区：
文献类型：
--
作者：
Wang Y;Ye X;Ding G;Xu F
植酸盐是土壤和植物种子中有机磷的主要贮存形式，而植物和单胃动物不能利用植酸盐中的磷。本研究将植酸酶基因phyA和appA分别与信号肽序列和CaMV 35 S启动子一起导入甘蓝型油菜（Brassica napus cv Westar）。选择了三个独立的转基因株系，来自phyA的P3和P11以及来自appA的a18。与野生型（WT）对照相比，三个转基因株系表现出显着更高的渗出植酸酶活性。石英砂培养试验表明，转基因油菜显著提高了植株的磷吸收量和生物量。土培试验表明，转基因株系P11和a18的种子产量分别比野生型提高了20.9%和59.9%。当植酸作为唯一的磷源时，种子中的磷积累量在P11和a18中分别比WT增加了20.6%和46.9%。P3系在种子中积累的P显著高于WT，而当植酸盐用作唯一P源时，种子产量没有显著差异。转基因油菜种子中植酸酶活性范围为1，138至1，605 U kg-1种子，而WT种子中未检测到植酸酶活性。此外，植酸含量在P11和a18种子显着低于野生型。这些结果介绍了一个机会，通过油菜的遗传操作，提高土壤和种子植酸磷的生物利用度，从而增加植物产量和单胃动物的磷营养。
Phytate is the major storage form of organic phosphorus in soils and plant seeds, and phosphorus (P) in this form is unavailable to plants or monogastric animals. In the present study, the phytase genes phyA and appA were introduced into Brassica napus cv Westar with a signal peptide sequence and CaMV 35S promoter, respectively. Three independent transgenic lines, P3 and P11 from phyA and a18 from appA, were selected. The three transgenic lines exhibited significantly higher exuded phytase activity when compared to wild-type (WT) controls. A quartz sand culture experiment demonstrated that transgenic Brassica napus had significantly improved P uptake and plant biomass. A soil culture experiment revealed that seed yields of transgenic lines P11 and a18 increased by 20.9% and 59.9%, respectively, when compared to WT. When phytate was used as the sole P source, P accumulation in seeds increased by 20.6% and 46.9% with respect to WT in P11 and a18, respectively. The P3 line accumulated markedly more P in seeds than WT, while no significant difference was observed in seed yields when phytate was used as the sole P source. Phytase activities in transgenic canola seeds ranged from 1,138 to 1,605 U kg–1 seeds, while no phytase activity was detected in WT seeds. Moreover, phytic acid content in P11 and a18 seeds was significantly lower than in WT. These results introduce an opportunity for improvement of soil and seed phytate-P bioavailability through genetic manipulation of oilseed rape, thereby increasing plant production and P nutrition for monogastric animals.
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影响因子：
5.2
作者：
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通讯作者：
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