Genetic Positioning of Centromeres through Half-Tetrad Analysis in Gynogenetic Diploid Families of the Zhikong Scallop (Chlamys farreri)
Genetic Positioning of Centromeres through Half-Tetrad Analysis in Gynogenetic Diploid Families of the Zhikong Scallop (Chlamys farreri)
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栉孔扇贝雌核发育二倍体家系半四分体分析的着丝粒遗传定位
DOI：
10.1007/s10126-012-9454-3
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发表时间：
2013-02
影响因子：
3
通讯作者：
Kong, Lingfeng
中科院分区：
文献类型：
--
作者：
Nie, Hongtao;Li, Qi;Zhao, Xuelin;Kong, Lingfeng
着丝粒作图是改进连锁图谱、研究交叉事件和了解减数分裂中交叉干扰的有力工具。从栉孔扇贝遗传图谱中筛选出90个微卫星标记，应用半四分体分析法对3个人工诱导的雌核发育家系进行了着丝粒定位。遗传分析表明，对照组合中90个微卫星位点均符合孟德尔遗传规律，而在减数分裂后代中有4.4%的微卫星位点出现分离偏离。第二次分裂分离频率（y）的变化范围为0.033 ~ 0.778，平均值为0.332，表明该物种存在部分交叉干扰。在42例标记在一个以上的家庭中分型的病例中观察到异源性，表明家庭之间的基因-着丝粒重组存在差异。着丝粒的位置与C. farreri核型，但标记之间的连锁和着丝粒图发生的顺序的差异。本研究为扇贝染色体组学研究提供了一个更完整、更丰富的遗传连锁图谱，也为进一步研究扇贝着丝粒的结构和功能奠定了基础。
Centromere mapping is a powerful tool for improving linkage maps, investigating crossover events, and understanding chiasma interference during meiosis. Ninety microsatellite markers selected across all linkage groups (LGs) from a previousChlamys farrerigenetic map were studied in three artificially induced meiogynogenetic families for centromere mapping by half-tetrad analysis. Inheritance analyses showed that all 90 microsatellite loci conformed to Mendelian inheritance in the control crosses, while 4.4 % of the microsatellite loci showed segregation departures from an expected 1:1 ratio of two homozygote classes in meiogynogenetic progeny. The second division segregation frequency (y) of the microsatellites ranged from 0.033 to 0.778 with a mean of 0.332, confirming the occurrence of partial chiasma interference in this species. Heterogeneity ofyis observed in one of 42 cases in which markers were typed in more than one family, suggesting variation in gene–centromere recombination among families. Centromere location was mostly in accordance with theC. farrerikaryotype, but differences in marker order between linkage and centromere maps occurred. Overall, this study makes the genetic linkage map a more complete and informative tool for genomic studies and it will also facilitate future research of the structure and function of the scallop centromeres.
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影响因子：
3.3
作者：
E. Volpe
通讯作者：
E. Volpe
尼罗罗非鱼（Oreochromis niloticus）中性别连接的AFLP标记的遗传和物理图。
影响因子：
3
作者：
Lee, Bo-Young;Coutanceau, Jean-Pierre;Ozouf-Costaz, Catherine;D'Cotta, Helena;Baroiller, Jean-Francois;Kocher, Thomas D.
通讯作者：
Kocher, Thomas D.
影响因子：
4.4
作者：
Guyomard, Rene;Mauger, Stephane;Tabet-Canale, Kamila;Martineau, Sylvain;Genet, Carine;Krieg, Francine;Quillet, Edwige
通讯作者：
Quillet, Edwige
影响因子：
3
作者：
Qi Li;A. Kijima
通讯作者：
Qi Li;A. Kijima
影响因子：
4.4
作者：
Foley BR;Rose CG;Rundle DE;Leong W;Moy GW;Burton RS;Edmands S
通讯作者：
Edmands S