Expression of the insulin-like growth factor (IGF) system in the bovine oviduct at oestrus and during early pregnancy.
Expression of the insulin-like growth factor (IGF) system in the bovine oviduct at oestrus and during early pregnancy.
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发情期和妊娠早期牛输卵管中胰岛素样生长因子（IGF）系统的表达。
作者：
PG Pushpakumara;RS Robinson;KJ Demmers;GE Mann;KD Sinclair;R. Webb;D. Wathes
早期哺乳动物胚胎体外发育可以通过与输卵管细胞共培养和添加胰岛素样生长因子（IGFs）来增强。本研究探讨了牛输卵管IGF系统的表达模式与发情后天数和胚胎存在与否的关系。输卵管采集自：（i）授精后第3天、第6天或第16天的66头未经产小母牛，以及（ii）发情周期第0天或第1天的10头非妊娠泌乳母牛。将输卵管盘绕，整个冷冻并切片用于原位杂交。通过放射自显影测定编码IGF-I、IGF-II、1型IGF受体（IGF-1 R）和IFG结合蛋白（IGFBP）-1、-3和-5的mRNA的表达模式。分别测量漏斗部、壶腹和峡部的粘膜层和肌肉层。没有测量的参数之间的小母牛有或没有胚胎的存在不同。编码IGF-I和IGF-1 R的mRNA存在于所有三个输卵管区域的粘膜和肌肉中，并且在第3天在小母牛中测量到IGF-I mRNA的最高值。IGF-II mRNA主要表达于肌壁。未检测到IGFBP-1 mRNA，而编码IGFBP-3和-5的mRNA在肌肉和粘膜中均表达。IGFBP-3的表达在母牛发情周期的第0天和第1天高于小母牛授精后第3天、第6天和第16天。IGFBP-5表达在第6天达到峰值。局部或全身产生的IGF受IGFBPs调节，可能直接作用于胚胎，或通过调节输卵管分泌物和肌肉活动间接作用于胚胎，以影响早期胚胎发育的成功。
Early mammalian embryo development in vitro can be enhanced by co-culture with oviductal cells and by the addition of insulin-like growth factors (IGFs). This study examined the expression patterns of the oviductal IGF system in cattle in relation to the number of days after oestrus and the presence or absence of embryos. Oviducts were collected from: (i) 66 nulliparous heifers on day 3, day 6 or day 16 after insemination and from (ii) ten non-pregnant, lactating cows on day 0 or day 1 of the oestrous cycle. Oviducts were coiled, frozen whole and sectioned for in situ hybridization. Expression patterns of mRNAs encoding IGF-I, IGF-II, type 1 IGF receptor (IGF-1R), and the IFG binding proteins (IGFBP)-1, -3 and -5 were determined from autoradiographs. Separate measurements were made for the mucosa and muscle layers of the infundibulum, ampulla and isthmus. None of the parameters measured differed between heifers with or without the presence of an embryo. mRNAs encoding IGF-I and IGF-1R were present in the mucosa and muscle of all three oviductal regions, and the highest value of IGF-I mRNA was measured in heifers on day 3. IGF-II mRNA was expressed predominantly in the muscle wall. IGFBP-1 mRNA was not detectable, whereas mRNAs encoding IGFBP-3 and -5 were expressed in both the muscle and mucosa. IGFBP-3 expression was higher in cows on day 0 and day 1 of the oestrous cycle than in heifers on day 3, day 6 and day 16 after insemination. A peak of IGFBP-5 expression was reached on day 6. Locally or systemically produced IGFs, regulated by IGFBPs, may act directly on the embryo or indirectly via modulation of oviductal secretions and muscular activity to influence the success of early embryo development.
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人输卵管中胰岛素样生长因子结合蛋白的鉴定。
影响因子：
6.7
作者：
Giudice,LC;Dsupin,BA;Irwin,JC;Eckert,RL
通讯作者：
Eckert,RL
影响因子：
20.3
作者：
John I. Jones;D. Clemmons
通讯作者：
John I. Jones;D. Clemmons
DOI：
--
发表时间：
1997-09
期刊：
Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy
影响因子：
--
作者：
S. Heyner
通讯作者：
S. Heyner
着床前小鼠输卵管生长因子、白介素-1、乳铁蛋白基因的表达及炎性白细胞分布分析。
影响因子：
3.6
作者：
Dalton,T;Kover,K;Dey,SK;Andrews,GK
通讯作者：
Andrews,GK
妊娠早期绵羊输卵管伞毛分泌状态、细胞高度和上皮衬里纤毛百分比的变化。
DOI：
10.1530/jrf.0.1060173
发表时间：
1996
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Murray,MK
通讯作者：
Murray,MK