Acute joint pathology and synovial inflammation is associated with increased intra-articular fracture severity in the mouse knee.
Acute joint pathology and synovial inflammation is associated with increased intra-articular fracture severity in the mouse knee.
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DOI：
10.1016/j.joca.2011.04.011
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发表时间：
2011-07
影响因子：
7
通讯作者：
Olson, S. A.
中科院分区：
文献类型：
--
作者：
Lewis, J. S.;Hembree, W. C.;Furman, B. D.;Tippets, L.;Cattel, D.;Huebner, J. L.;Little, D.;DeFrate, L. E.;Kraus, V. B.;Guilak, F.;Olson, S. A.
创伤后关节炎是一种常见的残疾原因，最常见和可预见的发生后，关节骨折。本研究的目的是在一种新的小鼠关节内骨折模型中检测骨折严重程度对急性关节病理学的影响。在成年雄性C57 BL/6小鼠中产生胫骨平台的低能量和高能量关节骨折（每组n=25）。在骨折后0、1、3、5和7天评估关节骨折严重程度对滑膜炎症、骨形态、游离骨折面积、软骨病理学、软骨细胞活力和全身细胞因子和生物标志物水平的急性影响。与对照肢体相比，关节内骨折严重程度的增加与滑膜和骨中更大的急性病理学相关，包括整体滑膜炎增加和关节周围骨密度和厚度降低。所施加的骨折能量与松解的皮质骨表面积的程度显著相关，表明粉碎程度更大。血清透明质酸（HA）浓度显着增加骨折后一天。虽然关节骨折显著降低软骨细胞活力，但骨折严重程度与软骨细胞活力、软骨变性或全身细胞因子和生物标志物水平之间没有关系。这项研究表明，关节骨折与软骨细胞活力丧失和全身生物标志物水平升高相关，关节内骨折严重程度增加与各种关节组织中急性关节病理学增加相关，包括滑膜炎症、皮质粉碎和骨形态学。进一步描述关节骨折后的早期事件有助于创伤后关节炎的治疗。
Post-traumatic arthritis is a frequent cause of disability and occurs most commonly and predictably after articular fracture. The objective of this investigation was to examine the effect of fracture severity on acute joint pathology in a novel murine model of intra-articular fracture. Low and high energy articular fractures (n=25 per group) of the tibial plateau were created in adult male C57BL/6 mice. The acute effect of articular fracture severity on synovial inflammation, bone morphology, liberated fracture area, cartilage pathology, chondrocyte viability, and systemic cytokines and biomarkers levels was assessed at 0, 1, 3, 5, and 7 days post-fracture. Increasing intra-articular fracture severity was associated with greater acute pathology in the synovium and bone compared to control limbs, including increased global synovitis and reduced periarticular bone density and thickness. Applied fracture energy was significantly correlated with degree of liberated cortical bone surface area, indicating greater comminution. Serum concentrations of hyaluronic acid (HA) were significantly increased one day post-fracture. While articular fracture significantly reduced chondrocyte viability, there was no relationship between fracture severity and chondrocyte viability, cartilage degeneration, or systemic levels of cytokines and biomarkers. This study demonstrates that articular fracture is associated with a loss of chondrocyte viability and increased levels of systemic biomarkers, and that increased intra-articular fracture severity is associated with increased acute joint pathology in a variety of joint tissues, including synovial inflammation, cortical comminution, and bone morphology. Further characterization of the early events following articular fracture could aid in the treatment of post-traumatic arthritis.
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影响因子：
2.8
作者：
Anderson, Donald D.;Mosqueda, Teresa;ThomaS, Thaddeus;Hermanson, Evan L.;Brown, Thomas D.;Marsh, J. Lawrence
通讯作者：
Marsh, J. Lawrence
DOI：
10.1097/01.blo.0000136653.48752.7c
发表时间：
2004-09-01
影响因子：
4.2
作者：
Borrelli, J;Zhu, Y;Silva, MJ
通讯作者：
Silva, MJ
影响因子：
5
作者：
Goldring, M B
通讯作者：
Goldring, M B
影响因子：
4.1
作者：
Beamer, WG;Donahue, LR;Baylink, DJ
通讯作者：
Baylink, DJ
DOI：
10.1097/00003086-200110001-00009
发表时间：
2001-10-01
影响因子：
4.2
作者：
D'Lima, DD;Hashimoto, S;Lotz, MK
通讯作者：
Lotz, MK