A specific high-performance liquid chromatography assay for dehydroascorbic acid shows an increased content in CLL lymphocytes.
A specific high-performance liquid chromatography assay for dehydroascorbic acid shows an increased content in CLL lymphocytes.
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脱氢抗坏血酸的特定高效液相色谱测定显示 CLL 淋巴细胞中的含量增加。
DOI：
10.1016/0003-2697(83)90309-3
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发表时间：
1983
影响因子：
2.9
通讯作者：
Silber,R
中科院分区：
文献类型：
--
作者：
Farber,CM;Kanengiser,S;Stahl,R;Liebes,L;Silber,R
A method for the assay of dehydroascorbic acid using high-performance liquid chromatography with uv detection is described. The dehydroascorbic acid is separated from ascorbic acid and reduced with dithiothreitol, and is then quantitated as ascorbic acid following rechromatography. Since as little as 22 pmol can be detected, sensitivity is at least 40-fold greater than that of other currently available procedures. This method was used to measure the level of dehydroascorbic acid in normal and chronic lymphocytic leukemia lymphocytes. A significantly higher concentration of dehydroascorbic acid was found in leukemic (21.80 ± 3.55 nmol 108cells , mean ± SE) than in normal lymphocytes (9.32 ± 1.15 nmol 108cells ) (P < 0.03). Analysis of extracts from normal B cell lymphocytes revealed comparable dehydroascorbic acid levels to unfractionated lymphocytes, indicating that the elevated level in chronic lymphocytic leukemia was not simply a reflection of the increased percentage of B lymphocytes in this disorder. These studies illustrate that the technique can be used to measure the dehydroascorbic acid content from sources where only scanty material is available or low levels are found.
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人血中性粒细胞、红细胞和淋巴细胞对脱氢坏血酸的吸收和减少 *
影响因子：
5.2
作者：
L. Stankova;D. Rigas;R. Bigley
通讯作者：
R. Bigley
血浆中L-抗坏血酸和L-脱氢抗坏血酸测定的改进方法。
DOI：
--
发表时间：
1980
期刊：
Clinica chimica acta; international journal of clinical chemistry
影响因子：
--
作者：
Okamura Masato
通讯作者：
Okamura Masato
通过高效液相色谱法鉴定和定量人淋巴细胞提取物中的抗坏血酸。
影响因子：
2.9
作者：
Liebes,LF;Kuo,S;Krigel,R;Pelle,E;Silber,R
通讯作者：
Silber,R
生物体液中的抗坏血酸分析。
影响因子：
2.9
作者：
Rose,RC;Nahrwold,DL
通讯作者：
Nahrwold,DL
影响因子：
2.9
作者：
CHATTERJEE, IB;BANERJEE, A
通讯作者：
BANERJEE, A