Characterization of Active Electrode Yield for Intracortical Arrays: Awake versus Anesthesia.
Characterization of Active Electrode Yield for Intracortical Arrays: Awake versus Anesthesia.
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DOI：
10.3390/mi13030480
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发表时间：
2022-03-20
期刊：
影响因子：
3.4
通讯作者：
Pancrazio JJ
中科院分区：
文献类型：
--
作者：
Sturgill B;Radhakrishna R;Thai TTD;Patnaik SS;Capadona JR;Pancrazio JJ
皮质内微电极阵列用于以单位分辨率记录神经信号，并且是研究脑功能和开发神经修复术的有前途的工具。目前正在进行研究，以提高这些探头的长期性能和可靠性，这些探头在植入后几个月内往往会下降或失效。尽管记录模式各不相同，但侧重于评估这些器械可靠性和性能的研究通常在麻醉下进行记录。然而，众所周知，异氟烷等麻醉剂会改变神经活动和电生理功能。因此，我们比较了植入雄性和雌性Sprague-Dawley大鼠运动皮层的探针在麻醉（2%异氟烷）和清醒条件下的神经记录性能。虽然与麻醉条件相比，清醒条件下的单单位尖峰率显著高出近600%，但我们发现手术后两周两种条件下的有源电极产率无差异。此外，麻醉下的信噪比更高，因为清醒记录中的噪声水平高出近50%，尽管清醒时可分辨的单个单位的峰间电压增加了14%。我们观察到，这些发现在慢性时间点也是相似的。我们的观察结果表明，无论是麻醉或清醒的记录是可以接受的研究评估慢性可靠性和性能的皮质内微电极阵列。
Intracortical microelectrode arrays are used for recording neural signals at single-unit resolution and are promising tools for studying brain function and developing neuroprosthetics. Research is being done to increase the chronic performance and reliability of these probes, which tend to decrease or fail within several months of implantation. Although recording paradigms vary, studies focused on assessing the reliability and performance of these devices often perform recordings under anesthesia. However, anesthetics—such as isoflurane—are known to alter neural activity and electrophysiologic function. Therefore, we compared the neural recording performance under anesthesia (2% isoflurane) followed by awake conditions for probes implanted in the motor cortex of both male and female Sprague-Dawley rats. While the single-unit spike rate was significantly higher by almost 600% under awake compared to anesthetized conditions, we found no difference in the active electrode yield between the two conditions two weeks after surgery. Additionally, the signal-to-noise ratio was greater under anesthesia due to the noise levels being nearly 50% greater in awake recordings, even though there was a 14% increase in the peak-to-peak voltage of distinguished single units when awake. We observe that these findings are similar for chronic time points as well. Our observations indicate that either anesthetized or awake recordings are acceptable for studies assessing the chronic reliability and performance of intracortical microelectrode arrays.
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