Look-up and look-down neurons in the mouse visual thalamus during freely moving exploration.
Look-up and look-down neurons in the mouse visual thalamus during freely moving exploration.
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DOI：
10.1016/j.cub.2022.07.049
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发表时间：
2022-09-26
期刊：
影响因子：
9.2
通讯作者：
Storchi, Riccardo
中科院分区：
文献类型：
--
作者：
Orlowska-Feuer, Patrycja;Ebrahimi, Aghileh S.;Zippo, Antonio G.;Petersen, Rasmus S.;Lucas, Robert J.;Storchi, Riccardo
视觉信息通过丘脑背外侧膝状体核（dLGN）到达皮层。dLGN活动受整体睡眠/觉醒状态和唤醒的调节，表明它不仅仅是一个被动的中继站。然而，其更具体的可视化集成的潜力在很大程度上是未开发的。我们解决了这个问题，通过开发强大的三维视频重建的小鼠头部和身体在自发探索配对的同时从dLGN神经元记录。对各种姿势和动作的无偏见评估揭示了神经元活动和少数行为参数之间的广泛耦合。特别是，与动物向上看/向下看相关的姿势与>50%的神经元的活动相关，这种影响的程度与全身运动（通常是运动）引起的影响相当。相比之下，丘脑活动与其他姿势或运动（例如，左/右头部和身体扭转）。重要的是，上下姿势和全身运动在很大程度上是独立的，并与神经元活动相关联。因此，尽管大多数单位在全身运动时兴奋，但有些单位在动物向上看时表现出最高的放电（“向上看”神经元），而其他单位在动物向下看时表现出最高的放电（“向下看”神经元）。这些结果是在黑暗中观察到的，因此代表了真正的行为调节，并在照明的竞技场中被放大。我们的研究结果表明，初级视觉丘脑，超越全球调制的睡眠/清醒状态，是潜在的参与特定的视觉整合，并揭示了两个不同的耦合之间的向上/向下的姿势和神经元活动。向上和向下的姿势与丘脑dLGN中的放电率相耦合向上/向下姿势的耦合在很大程度上独立于运动。耦合是多样的，并通过向上和向下的神经元来表达。发现在自由活动的小鼠中，向上和向下的姿势与丘脑dLGN中两个不同群体的兴奋相关，定义为向上和向下的神经元。这种效应在很大程度上独立于运动，存在于黑暗中，并在明视光下被放大。
Visual information reaches cortex via the thalamic dorsal lateral geniculate nucleus (dLGN). dLGN activity is modulated by global sleep/wake states and arousal, indicating that it is not simply a passive relay station. However, its potential for more specific visuomotor integration is largely unexplored. We addressed this question by developing robust 3D video reconstruction of mouse head and body during spontaneous exploration paired with simultaneous neuronal recordings from dLGN. Unbiased evaluation of a wide range of postures and movements revealed a widespread coupling between neuronal activity and few behavioral parameters. In particular, postures associated with the animal looking up/down correlated with activity in >50% neurons, and the extent of this effect was comparable with that induced by full-body movements (typically locomotion). By contrast, thalamic activity was minimally correlated with other postures or movements (e.g., left/right head and body torsions). Importantly, up/down postures and full-body movements were largely independent and jointly coupled to neuronal activity. Thus, although most units were excited during full-body movements, some expressed highest firing when the animal was looking up (“look-up” neurons), whereas others expressed highest firing when the animal was looking down (“look-down” neurons). These results were observed in the dark, thus representing a genuine behavioral modulation, and were amplified in a lit arena. Our results demonstrate that the primary visual thalamus, beyond global modulations by sleep/awake states, is potentially involved in specific visuomotor integration and reveal two distinct couplings between up/down postures and neuronal activity. Upward- and downward-facing postures are coupled to firing rate in thalamic dLGN The coupling to up/down postures is largely independent from locomotion The coupling is diverse and expressed via look-up and look-down neurons Look-up and look-down neuron types are stable across dark and photopic light Orlowska-Feuer et al. found that in freely moving mice, upward- and downward-facing postures are associated with excitation of two distinct populations in thalamic dLGN, defined as look-up and look-down neurons. This effect is largely independent from locomotion, is present in the dark, and is amplified under photopic light.
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影响因子：
16.2
作者：
Bennett C;Arroyo S;Hestrin S
通讯作者：
Hestrin S
DOI：
10.1002/cne.22223
发表时间：
2010-03-01
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Bickford ME;Slusarczyk A;Dilger EK;Krahe TE;Kucuk C;Guido W
通讯作者：
Guido W
影响因子：
3.2
作者：
Benjamin AS;Fernandes HL;Tomlinson T;Ramkumar P;VerSteeg C;Chowdhury RH;Miller LE;Kording KP
通讯作者：
Kording KP
影响因子：
9.8
作者：
Cao, Liyu;Haendel, Barbara
通讯作者：
Haendel, Barbara
影响因子：
9.2
作者：
Erisken, Sinem;Vaiceliunaite, Agne;Busse, Laura
通讯作者：
Busse, Laura