PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a Rat Model of Cerebral Ischemic-Reperfusion Injury.
PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a Rat Model of Cerebral Ischemic-Reperfusion Injury.
复制标题
PET显示大鼠脑缺血再灌注损伤模型经丹红注射液治疗后功能恢复
DOI：
10.1155/2014/430757
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Wang Z;Song F;Li J;Zhang Y;He Y;Yang J;Zhou H;Zhao T;Fu W;Xing P;Wan H;Tian M;Zhang H
本研究采用18氟脱氧葡萄糖正电子发射断层扫描（18 F-FDG-PET）技术观察丹红注射液（DHI）对大鼠脑缺血的保护作用。法将大鼠随机分为5组：假手术组（Sham）、缺血再灌注未处理组（IRU）、DHI-1组（DHI 1 mL/kg/d）、DHI-2组（DHI 2 mL/kg/d）、DHI-4组（DHI 4 mL/kg/d）。所有治疗组每天腹腔注射DHI，连续14天。从脑梗死体积、神经功能、脑葡萄糖代谢等方面评价治疗效果。酶联免疫吸附法（ELISA）检测TNF-α、IL-1β的表达。通过免疫组织化学方法评估成熟神经元标记物（NeuN）、胶质细胞标记物（GFAP）、血管密度因子（vWF）和葡萄糖转运蛋白1（GLUT 1）的水平。结果与IRU组相比，DHI治疗组大鼠脑梗死体积和促炎细胞因子水平呈剂量依赖性减少，神经功能改善，脑葡萄糖代谢恢复。免疫组化结果显示，DHI治疗后梗死灶周围神经元、胶质细胞和血管数量明显增加，葡萄糖利用率恢复。结论本研究通过18F-FDG的micro-PET成像证实了DHI治疗后的代谢恢复以及DHI在脑缺血再灌注损伤大鼠模型中的神经保护作用。
This study aimed to investigate neuroprotection of Danhong injection (DHI) in a rat model of cerebral ischemia using 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET). Method. Rats were divided into 5 groups: sham group, ischemia-reperfusion untreated (IRU) group, DHI-1 group (DHI 1 mL/kg/d), DHI-2 group (DHI 2 mL/kg/d), and DHI-4 group (DHI 4 mL/kg/d). AII the treated groups were intraperitoneally injected with DHI daily for 14 days. The therapeutic effects in terms of cerebral infarct volume, neurological function, and cerebral glucose metabolism were evaluated. Expression of TNF-α and IL-1β was detected with enzyme-linked immunosorbent assay (ELISA). Levels of mature neuronal marker (NeuN), glial marker (GFAP), vascular density factor (vWF), and glucose transporter 1 (GLUT1) were assessed by immunohistochemistry. Results. Compared with the IRU group, rats treated with DHI showed dose dependent reductions in cerebral infarct volume and levels of proinflammatory cytokines, improvement of neurological function, and recovery of cerebral glucose metabolism. Meanwhile, the significantly increased numbers of neurons, gliocytes, and vessels and the recovery of glucose utilization were found in the peri-infarct region after DHI treatment using immunohistochemical analysis. Conclusion. This study demonstrated the metabolic recovery after DHI treatment by micro-PET imaging with 18F-FDG and the neuroprotective effects of DHI in a rat model of cerebral ischemic-reperfusion injury.
登录
查看更多内容
临时[校正]脑缺血会导致星形细胞末端肿胀，从而压缩微丝并导致延迟[校正]局灶性皮质梗塞。
DOI：
10.1038/jcbfm.2010.97
发表时间：
2011-01
期刊：
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1155/2013/507091
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Li JH;Lu J;Zhang H
通讯作者：
Zhang H
影响因子：
5.3
作者：
Ohab, John J.;Fleming, Sheila;Carmichael, S. Thomas
通讯作者：
Carmichael, S. Thomas
影响因子：
8.3
作者：
GARCIA, JH;WAGNER, S;HU, XJ
通讯作者：
HU, XJ
影响因子：
3.6
作者：
Prabhakar, Orsu
通讯作者：
Prabhakar, Orsu