The effects of aging on hypoglossal motoneurons in rats.
The effects of aging on hypoglossal motoneurons in rats.
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DOI：
10.1007/s00455-008-9169-9
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发表时间：
2009-03
期刊：
影响因子：
2.6
通讯作者：
Behan, Mary
中科院分区：
文献类型：
--
作者：
Schwarz, Emilie C.;Thompson, Jodi M.;Connor, Nadine P.;Behan, Mary
衰老会导致大脑和脊髓某些区域神经元细胞体的损失和神经元尺寸的减小。人们认为脊髓中运动神经元的损失会导致肌肉质量和力量随着年龄的增长而逐渐下降（肌肉减少症）。吞咽障碍是老年人的一个重大临床问题；然而，支配吞咽肌肉的颅运动神经元与年龄相关的变化尚未得到充分研究。我们的目的是确定脑干舌下核是否发生与年龄相关的改变。如果存在，这些变化可能有助于解释老年大鼠中报道的神经肌肉接头的变化和舌肌收缩特性的变化。我们假设随着年龄的增长，运动神经元会减少，神经元大小和与每个舌下运动神经元相关的初级树突数量也会减少。使用神经元标记物 NeuN 对年轻（9-10 个月）、中年（24-25 个月）和老年（32-33 个月）雄性 F344/BN 大鼠舌下核中的神经元进行可视化。通过将霍乱毒素 β 注射到舌头的颏舌肌中来逆行标记舌下运动神经元，并使用免疫细胞化学进行可视化。结果表明，舌下运动神经元的初级树突数量随着年龄的增长而显着减少，而舌下运动神经元的数量或大小没有发现与年龄相关的变化。初级树突的损失可能会减少突触输入的数量，从而损害功能。
Aging can result in a loss of neuronal cell bodies and a decrease in neuronal size in some regions of the brain and spinal cord. Motoneuron loss in the spinal cord is thought to contribute to the progressive decline in muscle mass and strength that occurs with age (sarcopenia). Swallowing disorders represent a large clinical problem in elderly persons; however, age-related alterations in cranial motoneurons that innervate muscles involved in swallowing have been understudied. We aimed to determine if age-related alterations occurred in the hypoglossal nucleus in the brainstem. If present, these changes might help explain alterations at the neuromuscular junction and changes in the contractile properties of tongue muscle that have been reported in older rats. We hypothesized that with increasing age, there would be a loss of motoneurons and a reduction in neuronal size and the number of primary dendrites associated with each hypoglossal motoneuron. Neurons in the hypoglossal nucleus were visualized with the neuronal marker NeuN in young (9–10 months), middle-aged (24–25 months), and old (32–33 months) male F344/BN rats. Hypoglossal motoneurons were retrograde labeled with injections of Cholera Toxin β into the genioglossus muscle of the tongue and visualized using immunocytochemistry. Results indicated that the number of primary dendrites of hypoglossal motoneurons decreased significantly with age, while no age-associated changes were found in the number or size of hypoglossal motoneurons. Loss of primary dendrites could reduce the number of synaptic inputs and thereby impair function.
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影响因子：
3.3
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FRONTERA, WR;MEREDITH, CN;EVANS, WJ
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作者：
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