Rapid identification of spinal ventral and dorsal roots using a quartz crystal microbalance.
Rapid identification of spinal ventral and dorsal roots using a quartz crystal microbalance.
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DOI：
10.3969/j.issn.1673-5374.2013.08.002
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发表时间：
2013-03-15
影响因子：
6.1
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Sui T;Que J;Kong D;Xie H;Wang D;Shi K;Cao X;Li X
神经束的快速准确识别是神经吻合成功的关键。利用脊髓腹根和背根之间乙酰胆碱酯酶含量的差异，我们发展了一种新的石英晶体微天平方法，根据乙酰胆碱酯酶抗体的反应性来区分这两种神经。将乙酰胆碱酯酶抗体固定在石英晶体微天平的电极表面，与样品溶液中的乙酰胆碱酯酶反应。形成的抗原和抗体复合体加到电极的质量上，引起电极频率的变化。通过频率的变化来区分脊神经腹根和背根。腹根和背根切成1~2 mm长的节段，浸泡在250μ的L缓冲液中。将乙酰胆碱酯酶抗体固定在石英晶体微天平金电极表面。结果表明，在10min内，脊髓腹根和背根均引起频率变化，但腹根引起的频率变化显著高于背根引起的频率变化。牛血清白蛋白或PBS不能引起上述变化。这些结果表明，石英晶体微天平传感器可以作为一种快速、高灵敏度和准确的检测工具，用于术中快速识别脊神经根。
The fast and accurate identification of nerve tracts is critical for successful nerve anastomosis. Taking advantage of differences in acetylcholinesterase content between the spinal ventral and dorsal roots, we developed a novel quartz crystal microbalance method to distinguish between these nerves based on acetylcholinesterase antibody reactivity. The acetylcholinesterase antibody was immobilized on the electrode surface of a quartz crystal microbalance and reacted with the acetylcholinesterase in sample solution. The formed antigen and antibody complexes added to the mass of the electrode inducing a change in frequency of the electrode. The spinal ventral and dorsal roots were distinguished by the change in frequency. The ventral and dorsal roots were cut into 1 to 2-mm long segments and then soaked in 250 μL PBS. Acetylcholinesterase antibody was immobilized on the quartz crystal microbalance gold electrode surface. The results revealed that in 10 minutes, both spinal ventral and dorsal roots induced a frequency change; however, the frequency change induced by the ventral roots was notably higher than that induced by the dorsal roots. No change was induced by bovine serum albumin or PBS. These results clearly demonstrate that a quartz crystal microbalance sensor can be used as a rapid, highly sensitive and accurate detection tool for the quick identification of spinal nerve roots intraoperatively.
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影响因子：
2.9
作者：
GRUBER, H;ZENKER, W
通讯作者：
ZENKER, W
影响因子：
12.6
作者：
Kim, Dong-Min;Rahman, Md. Aminur;Shim, Yoon-Bo
通讯作者：
Shim, Yoon-Bo
影响因子：
7.4
作者：
OKAHATA, Y;ENNA, G;EBATO, H
通讯作者：
EBATO, H
DOI：
10.1097/01.bth.0000134712.83301.0a
发表时间：
2004-09-01
影响因子：
--
作者：
Hattori, Yasunori;Doi, Kazuteru
通讯作者：
Doi, Kazuteru
影响因子：
12.6
作者：
Campbell, GA;Mutharasan, R
通讯作者：
Mutharasan, R