Low-magnitude high-frequency vibration inhibits RANKL-induced osteoclast differentiation of RAW264.7 cells.
Low-magnitude high-frequency vibration inhibits RANKL-induced osteoclast differentiation of RAW264.7 cells.
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低强度高频振动抑制 RANKL 诱导的 RAW264.7 细胞破骨细胞分化
DOI：
10.7150/ijms.4838
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发表时间：
2012
影响因子：
3.6
通讯作者：
Chen JT
中科院分区：
文献类型：
--
作者：
Wu SH;Zhong ZM;Chen JT
破骨细胞是调节骨量的关键参与者。低幅高频振动（LMHFV）已被发现是合成代谢的骨在体内。本研究旨在探讨LMHFV对体外破骨细胞分化的影响。在存在核因子-κ B配体受体激活剂（RANKL）的情况下，小鼠单核细胞系RAW 264.7细胞在第-1天用或不用LMHFV（45 Hz，0.3 g）处理15 min。抗酒石酸酸性磷酸酶（TRAP）阳性多核细胞（MNCs）和肌动蛋白环的形成进行了评价。采用真实的时间PCR分析破骨细胞特异性基因如组织蛋白酶K、基质金属肽酶9（MMP-9）和TRAP的表达。Western blot检测破骨细胞特异性转录因子c-Fos的表达。结果发现，LMHFV能显著减少RANKL诱导的TRAP阳性细胞数（P<0.01），并抑制肌动蛋白环的形成。组织蛋白酶K、MMP-9和TRAP的mRNA表达在LMHFV干预后明显下调（P均<0.001）。此外，LMHFV还能抑制RANKL处理的RAW264.7细胞c-Fos蛋白的表达（P<0.05）。结果表明，LMHFV能抑制RANKL诱导的RAW264.7细胞破骨细胞分化，这为LMHFV对骨合成代谢的影响提供了新的认识。
Osteoclasts are the key participants in regulation of bone mass. Low-magnitude high-frequency vibration (LMHFV) has been found to be anabolic to bone in vivo. This study aimed to investigate the effect of LMHFV on osteoclast differentiation in vitro. Murine monocyte cell line RAW264.7 cells in the presence of receptor activator of nuclear factor-kappaB ligand (RANKL) were treated with or without LMHFV at 45 Hz (0.3 g) for 15 min day−1. Tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells (MNCs) and actin ring formation were evaluated. Expression of the osteoclast-specific genes, such as cathepsin K, matrix metallopeptidase-9 (MMP-9) and TRAP, were analyzed using real time-PCR. c-Fos, an osteoclast-specific transcription factor, was determined using Western blot. We found that LMHFV significantly decreased the number of RANKL-induced TRAP-positive MNCs (P<0.01), and inhibited the actin ring formation. The mRNA expression of the cathepsin K, MMP-9 and TRAP were down-regulated by LMHFV intervention (all P<0.001). Furthermore, LMHFV also inhibited the expression of c-Fos protein in the RANKL-treated RAW264.7 cells (P<0.05). Our results suggest that LMHFV can inhibit the RANKL-induced osteoclast differentiation of RAW264.7 cells, which give some new insight into the anabolic effects of LMHFV on bone.
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影响因子：
2.8
作者：
Lau, Esther;Lee, W. David;Li, Jason;Xiao, Andrew;Davies, John E.;Wu, Qianhong;Wang, Liyun;You, Lidan
通讯作者：
You, Lidan
影响因子：
3.9
作者：
Kitami, Satoshi;Tanaka, Hideki;Maeno, Masao
通讯作者：
Maeno, Masao
影响因子：
4.1
作者：
Lau, Esther;Al-Dujaili, Saja;Guenther, Axel;Liu, Dawei;Wang, Liyun;You, Lidan
通讯作者：
You, Lidan
影响因子：
4.1
作者：
Kim, Chi Hyun;You, Lidan;Jacobs, Christopher R.
通讯作者：
Jacobs, Christopher R.
DOI：
10.1073/pnas.87.18.7260
发表时间：
1990-09-01
影响因子：
11.1
作者：
UDAGAWA, N;TAKAHASHI, N;SUDA, T
通讯作者：
SUDA, T