Effect of low-magnitude, high-frequency vibration on osteogenic differentiation of rat mesenchymal stromal cells.
Effect of low-magnitude, high-frequency vibration on osteogenic differentiation of rat mesenchymal stromal cells.
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DOI：
10.1002/jor.21334
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发表时间：
2011-07
影响因子：
2.8
通讯作者：
You, Lidan
中科院分区：
文献类型：
--
作者：
Lau, Esther;Lee, W. David;Li, Jason;Xiao, Andrew;Davies, John E.;Wu, Qianhong;Wang, Liyun;You, Lidan
关键词：
全身振动（WBV），由一个低幅度，高频率（LMHF）的信号，已被发现是合成代谢的骨在体内，这可能是通过改变间充质基质细胞（MSC）的谱系承诺。在这里，我们研究了LMHF振动对大鼠骨髓源性间充质干细胞（rMSCs）在体外系统的影响。在成骨诱导培养基的存在下，我们将rMSCs以0.3g和60 Hz反复进行（六次）1小时的振动。通过检查细胞增殖、碱性磷酸酶（ALP）活性、各种成骨细胞相关标志物（ALP、Runx 2、osterix、I型胶原α 1、骨唾液蛋白、骨桥蛋白和骨钙素）的mRNA表达以及基质矿化，评估了负载和非负载条件下rMSCs的成骨分化。我们观察到LMHF振动并没有增强rMSCs的成骨分化。令人惊讶的是，我们发现成骨细胞形成所必需的转录因子osterix的mRNA水平降低，基质矿化受到抑制。我们的研究结果表明，LMHF振动可能发挥其合成代谢的影响，在体内通过mechanosensing的细胞类型不同的骨髓间充质干细胞。
Whole body vibration (WBV), consisting of a low-magnitude, high-frequency (LMHF) signal, has been found to be anabolic to bone in vivo, which may act through alteration of the lineage commitment of mesenchymal stromal cells (MSC). Here, we investigated the effect of LMHF vibration on rat bone marrow-derived MSCs (rMSCs) in an in vitro system. We subjected rMSCs to repeated (six) bouts of 1-hour vibration at 0.3g and 60 Hz in the presence of osteogenic induction medium. The osteogenic differentiation of rMSCs under the loaded and non-loaded conditions was assessed by examining cell proliferation, alkaline phosphatase (ALP) activity, mRNA expression of various osteoblast-associated markers (ALP, Runx2, osterix, collagen type I alpha 1, bone sialoprotein, osteopontin, and osteocalcin), as well as matrix mineralization. We observed that LMHF vibration did not enhance the osteogenic differentiation of rMSCs. Surprisingly, we found that the mRNA level of osterix, a transcription factor necessary for osteoblast formation, was decreased, and matrix mineralization was inhibited. Our findings suggest that LMHF vibration may exert its anabolic effects in vivo via mechanosensing of a cell type different from MSCs.
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影响因子：
4.8
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.0708467104
发表时间：
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通讯作者：
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影响因子：
4.1
作者：
Lau, Esther;Al-Dujaili, Saja;Guenther, Axel;Liu, Dawei;Wang, Liyun;You, Lidan
通讯作者：
You, Lidan